Lee, Kyung Hyun Jeong, Sunjoo Yang, Eun Gyung Park, Yong-Keun Yu, Jaehoon 2024-01-21T00:03:02Z 2024-01-21T00:03:02Z 2021-08-31 2007-12-15 0968-0896 https://pubs.kist.re.kr/handle/201004/133883 The generation of molecules that selectively recognize specific conformations of a protein is an important component of the elucidation protein function. We have used SELEX (Systematic Evolution of Ligands by EXponential enrichment) technology to produce aptamers that bind in a conformationally selective manner to calsenilin, which involved in Ca2+-mediated apoptotic signaling. Since the conformations of calsenilin are quite different in the presence and absence of Ca2+, aptamers were selected against the dimeric protein both under calcium-bound and calcium-free conditions. We have found that aptamer-12 selectively binds to the dimeric form of the protein in the presence of calcium ion, while the binding of aptamer-2 does not discriminate between the Ca2+ bound and unbound protein. Data obtained from biochemical and biophysical experiments suggest that a dominant conformation of calcium-bound calsenilin exists in one dominant conformation and that one aptamer can be generated to recognize this conformation. In addition, observation made in this effort that aptamers selected against the two different conformations of calsenilin have different characteristics suggest that aptamers can serve as a plausible tool for recognizing various conformations of proteins, even those caused by interactions with small molecules or ions such as Ca2+. (c) 2007 Elsevier Ltd. All rights reserved. English PERGAMON-ELSEVIER SCIENCE LTD METAL-BINDING PROPERTIES CALSENILIN/DREAM/KCHIP3 EXPRESSION ANTIBODIES DISEASE An RNA aptamer that recognizes a specific conformation of the protein calsenilin Article 10.1016/j.bmc.2007.09.013 1 BIOORGANIC & MEDICINAL CHEMISTRY, v.15, no.24, pp.7545 - 7552 BIOORGANIC & MEDICINAL CHEMISTRY 15 24 7545 7552 scie scopus 000253489200004 2-s2.0-35348893331 Biochemistry & Molecular Biology Chemistry, Medicinal Chemistry, Organic Biochemistry & Molecular Biology Pharmacology & Pharmacy Chemistry Article METAL-BINDING PROPERTIES CALSENILIN/DREAM/KCHIP3 EXPRESSION ANTIBODIES DISEASE calsenilin conformation in vitro selection RNA aptamer SELEX