Kim, Choong Lee, In Hye Lee, Kangsun Ryu, Sung Shin Lee, Sang Ho Lee, Kyu-Jung Lee, Jinwoo Kang, Ji Yoon Kim, Tae Song 2024-01-21T00:04:20Z 2024-01-21T00:04:20Z 2021-09-02 2007-12 0916-8451 https://pubs.kist.re.kr/handle/201004/133944 A multi-well chip (MWC) is described by which mouse embryonic carcinoma (EC) stem cells form a comparatively more rapid and uniform embryoid body (EB) over the conventional hanging drop (HD) method. The newly developed MWC consists of an array of extruded through-holes, each of which holds a droplet of the cell suspension. The study found that the small curvature radius of the droplet in the MWC improved the EB formation rate of a hanging drop from 70% to 98%. Furthermore, the EBs formed by the MWC were uniformly round in shape regardless of the number of suspended cells ranging from 0.5 x 10(3) to 20 x 10(3). The ratio of beating colonies from the MWC was over 2-fold larger than that from HD. The experiments demonstrate that the MWC will be a valuable experimental tool for robust and reproducible EB-based differentiation of a defined number of ES cells. English TAYLOR & FRANCIS LTD IN-VITRO DIFFERENTIATION CARDIOMYOCYTES EFFICIENCY CULTURE BODIES Multi-well chip for forming a uniform embryoid body in a tiny droplet with mouse embryonic stem cells Article 10.1271/bbb.70384 1 BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, v.71, no.12, pp.2985 - 2991 BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 71 12 2985 2991 scie scopus 000252024400015 2-s2.0-37649016589 Biochemistry & Molecular Biology Biotechnology & Applied Microbiology Chemistry, Applied Food Science & Technology Biochemistry & Molecular Biology Biotechnology & Applied Microbiology Chemistry Food Science & Technology Article IN-VITRO DIFFERENTIATION CARDIOMYOCYTES EFFICIENCY CULTURE BODIES embryoid body multi-well chip hanging drop differentiation