Hwang, Kyo Seon Lee, Sang-Myung Eom, Kilho Lee, Jeong Hoon Lee, Yoon-Sik Park, Jung Ho Yoon, Dae Sung Kim, Tae Song 2024-01-21T00:04:51Z 2024-01-21T00:04:51Z 2021-09-02 2007-11-30 0956-5663 https://pubs.kist.re.kr/handle/201004/133968 We report the nanomechanical microcantilevers operated in vibration modes (oscillation) with use of RNA aptamers as receptor molecules for label-free detection of hepatitis C virus (HCV) helicase. The nanomechanical detection principle is that the ligand-receptor binding on the microcantilever surface induces the dynamic response change of microcantilevers. We implemented the label-free detection of HCV helicase in the low concentration as much as 100 pg/ml from measuring the dynamic response change of microcantilevers. Moreover, from the recent studies showing that the ligand-receptor binding generates the surface stress on the microcantilever, we estimate the surface stress, on the oscillating microcantilevers, induced by ligand-receptor binding, i.e. binding between HCV helicase and RNA aptamer. In this article, it is suggested that the oscillating microcantilevers with use of RNA aptamers as receptor molecules may enable one to implement the sensitive label-free detection of very small amount of small-scale proteins. (c) 2007 Elsevier B.V. All rights reserved. English ELSEVIER ADVANCED TECHNOLOGY ATOMIC-FORCE MICROSCOPE RESONANT-FREQUENCY SHIFT C-REACTIVE PROTEIN DNA-MOLECULES IN-VITRO CANTILEVER SELECTION VIRUS ARRAY ASSAY Nanomechanical microcantilever operated in vibration modes with use of RNA aptamer as receptor molecules for label-free detection of HCV helicase Article 10.1016/j.bios.2007.05.006 1 BIOSENSORS & BIOELECTRONICS, v.23, no.4, pp.459 - 465 BIOSENSORS & BIOELECTRONICS 23 4 459 465 scie scopus 000251120200002 2-s2.0-35348812686 Biophysics Biotechnology & Applied Microbiology Chemistry, Analytical Electrochemistry Nanoscience & Nanotechnology Biophysics Biotechnology & Applied Microbiology Chemistry Electrochemistry Science & Technology - Other Topics Article ATOMIC-FORCE MICROSCOPE RESONANT-FREQUENCY SHIFT C-REACTIVE PROTEIN DNA-MOLECULES IN-VITRO CANTILEVER SELECTION VIRUS ARRAY ASSAY nanomechanics microcantilever RNA aptamer HCV helicase resonant frequency