Llinas, Rodolfo R. Choi, Soonwook Urbano, Francisco J. Shin, Hee-Sup 2024-01-21T00:05:11Z 2024-01-21T00:05:11Z 2021-09-02 2007-11-06 0027-8424 https://pubs.kist.re.kr/handle/201004/133984 Thalamocortical in vivo and in vitro function was studied in mice lacking P/Q-type calcium channels (Ca(v)2.1), in which N-type calcium channels (Ca(v)2.2) supported central synaptic transmission. Unexpectedly, in vitro patch recordings from thalamic neurons demonstrated no gamma-band subthreshold oscillation, and voltage-sensitive dye imaging demonstrated an absence of cortical gamma-band-dependent columnar activation involving cortical inhibitory interneuron activity. In vivo electroencephalogram recordings showed persistent absence status and a dramatic reduction of gamma-band activity. Pharmacological block of T-type calcium channels (Ca(v)3), although not noticeably affecting normal control animals, left the knockout mice in a coma-like state. Hence, although N-type calcium channels can rescue P/Q-dependent synaptic transmission, P/Q calcium channels are essential in the generation of gamma-band activity and resultant cognitive function. English NATL ACAD SCIENCES VOLTAGE-ACTIVATED CURRENTS CENTRAL-NERVOUS-SYSTEM CALCIUM-CHANNEL CA2+ CHANNELS SYNAPTIC-TRANSMISSION NEUROTRANSMITTER RELEASE TRANSMITTER RELEASE NEURONS MOUSE FREQUENCY gamma-band deficiency and abnormal thalamocortical activity in P/Q-type channel mutant mice Article 10.1073/pnas.0707945104 1 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, v.104, no.45, pp.17819 - 17824 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 104 45 17819 17824 scie scopus 000250897600050 2-s2.0-36749085918 Multidisciplinary Sciences Science & Technology - Other Topics Article VOLTAGE-ACTIVATED CURRENTS CENTRAL-NERVOUS-SYSTEM CALCIUM-CHANNEL CA2+ CHANNELS SYNAPTIC-TRANSMISSION NEUROTRANSMITTER RELEASE TRANSMITTER RELEASE NEURONS MOUSE FREQUENCY 1-octanol patch clamp VSDI electroencephalogram calcium channel