Kim, Taeoh Kim, Sang Jin Kim, Kyunggon Kang, Un-Beom Lee, Cheolju Park, Kyong Soo Yu, Hyeong Gon Kim, Youngsoo 2024-01-21T00:05:36Z 2024-01-21T00:05:36Z 2021-09-02 2007-11 1615-9853 https://pubs.kist.re.kr/handle/201004/134003 Diabetes can lead to serious microvascular complications like proliferative diabetic retinopathy (PDR), which is the leading cause of blindness in adults. The proteomic changes that occur during PDR cannot be measured in the human retina for ethical reasons, but could be reflected by proteomic changes in vitreous humor. Thus, we considered that comparisons between the proteome profiles of the vitreous humors of PDR and nondiabetic controls could lead to the discovery of novel pathogenic proteins and clinical biomarkers. In this study, the authors used several proteomic methods to comprehensively examine vitreous humor proteomes of PDR patients and nondiabetic controls. These methods included immunoaffinity subtraction (IS)/2-DE/ MALDI-MS, nano-LC-MALDI-MS/MS, and nano-LC-ESI-MS/MS. The identified proteins were subjected to the Trans-Proteomic Pipeline validation process. Resultantly, 531 proteins were identified, i.e., 415 and 346 proteins were identified in PDR and nondiabetic control vitreous humor samples, respectively, and of these 5 3 1 proteins, 240 were identified for the first time in this study. The PDR vitreous proteome was also found to contain many proteins possibly involved in the pathogenesis of PDR. The proteins described provide the most comprehensive proteome listing in the vitreous humor samples of PDR and nondiabetic control patients. English WILEY ENDOTHELIAL GROWTH-FACTOR EPITHELIUM-DERIVED FACTOR RENIN-ANGIOTENSIN SYSTEM VASCULAR-PERMEABILITY HEPATOCYTE GROWTH EYE DISEASE MASS-SPECTROMETRY SOLUBLE-PROTEINS CELL MIGRATION MULLER CELLS Profiling of vitreous proteomes from proliferative diabetic retinopathy and nondiabetic patients Article 10.1002/pmic.200700745 1 PROTEOMICS, v.7, no.22, pp.4203 - 4215 PROTEOMICS 7 22 4203 4215 scie scopus 000251293000018 2-s2.0-36549073658 Biochemical Research Methods Biochemistry & Molecular Biology Biochemistry & Molecular Biology Article ENDOTHELIAL GROWTH-FACTOR EPITHELIUM-DERIVED FACTOR RENIN-ANGIOTENSIN SYSTEM VASCULAR-PERMEABILITY HEPATOCYTE GROWTH EYE DISEASE MASS-SPECTROMETRY SOLUBLE-PROTEINS CELL MIGRATION MULLER CELLS clinical proteomics diabetes diabetic retinopathy vitreous humor