Ahn, Woo-Sung Ahn, Ji-Young Jung, Chan-Hun Hwang, Kwang Yeon Kim, Eunice Eunkyeong Kim, Joon Im, Hana Kim, Jin-Oh Yu, Myeong-Hee Lee, Cheolju 2024-01-21T00:30:19Z 2024-01-21T00:30:19Z 2021-08-31 2007-11 1017-7825 https://pubs.kist.re.kr/handle/201004/134024 We have developed a robotic system for an automated parallel cell cultivation process that enables screening of induction parameters for the soluble expression of recombinant protein. The system is designed for parallelized and simultaneous cultivation of up to 24 different types of cells or a single type of cell at 24 different conditions. Twenty-four culture vessels of about 200 ml are arranged in four columns x six rows. The system is equipped with four independent thermostated waterbaths, each of which accommodates six culture vessels. A two-channel liquid handier is attached in order to distribute medium from the reservoir to the culture vessels, to transfer seed or other reagents, and to take an aliquot from the growing cells. Cells in each vessel are agitated and aerated by sparging filtered air. We tested the system by growing Escherichia coli BL21(DE3) cells harboring a plasmid for a model protein, and used it in optimizing protein expression conditions by varying the induction temperature and the inducer concentration. The results revealed the usefulness of our custom-made cell cultivation robot in screening optimal conditions for the expression of soluble proteins. English KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY BIOPROCESS DESIGN HTBD ESCHERICHIA-COLI STRUCTURAL GENOMICS RECOMBINANT PROTEINS MICROTITER PLATES THROUGHPUT PROTEOMICS PURIFICATION GROWTH YIELD Optimization of expression conditions for soluble protein by using a robotic system of multi-culture vessels Article 1 JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.17, no.11, pp.1868 - 1874 JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY 17 11 1868 1874 scie scopus kci ART001207370 000251242500018 2-s2.0-36949021085 Biotechnology & Applied Microbiology Microbiology Biotechnology & Applied Microbiology Microbiology Article BIOPROCESS DESIGN HTBD ESCHERICHIA-COLI STRUCTURAL GENOMICS RECOMBINANT PROTEINS MICROTITER PLATES THROUGHPUT PROTEOMICS PURIFICATION GROWTH YIELD parallel culture high throughput soluble protein robotic system