Park, Kyeongsoon Kim, Jong-Ho Nam, Yun Sik Lee, Seulki Nam, Hae Yun Kim, Kwangmeyung Park, Jae Hyung Kim, In-San Choi, Kuiwon Kim, Sang Yoon Kwon, Ick Chan 2024-01-21T00:30:47Z 2024-01-21T00:30:47Z 2021-08-31 2007-10-08 0168-3659 https://pubs.kist.re.kr/handle/201004/134046 To improve the in vivo tumor targeting characteristics of polymeric nanoparticles, three glycol chitosan (GC-20 kDa, GC-100 kDa, and GC-250 kDa) derivatives with different molecular weights were modified with cholanic acid at the same molar ratio, The resulting amphiphilic glycol chitosan-cholanic acid conjugates self-assembled to form glycol chitosan nanoparticles (GC-20 kDa-NP, GC-100 kDa-NP, and GC-250 kDa-NP) under aqueous conditions. The physicochemical properties of all three glycol chitosan nanoparticles, including degree of substitution with cholanic acid, surface charge, particle size and in vitro stability, were similar regardless of molecular weight. In vivo tissue distribution, time-dependent excretion, and tumor accumulation of glycol chitosan nanoparticles labeled with the near-infrared (NIR) fluorophore, Cy5.5, were monitored in SCC7 tumor-bearing mice, using NIR fluorescence imaging systems. Glycol chitosan nanoparticles displayed prolonged blood circulation time, decreased time-dependent excretion from the body, and elevated tumor accumulation with increasing polymer molecular weight. The results collectively suggest that high molecular weight glycol chitosan nanoparticles remain for longer periods in the blood circulation, leading to increased accumulation at the tumor site. Accordingly, we propose that enhanced tumor targeting by high molecular weight glycol chitosan nanoparticles is related to better in vivo stability, based on a pharmacokinetic improvement in blood circulation time. (c) 2007 Elsevier B.V. All rights reserved. English ELSEVIER MACROMOLECULAR THERAPEUTICS ACID BIODISTRIBUTION LIPOSOMES CARRIERS Effect of polymer molecular weight on the tumor targeting characteristics of self-assembled glycol chitosan nanoparticles Article 10.1016/j.jconrel.2007.04.009 1 JOURNAL OF CONTROLLED RELEASE, v.122, no.3, pp.305 - 314 JOURNAL OF CONTROLLED RELEASE 122 3 305 314 scie scopus 000250318800013 2-s2.0-34548623990 Chemistry, Multidisciplinary Pharmacology & Pharmacy Chemistry Pharmacology & Pharmacy Article; Proceedings Paper MACROMOLECULAR THERAPEUTICS ACID BIODISTRIBUTION LIPOSOMES CARRIERS glycol chitosan nanoparticle polymer molecular weight tissue distribution tumor targeting