Jung, Byung Hwa Rezk, Naser L. Bridges, Arlene S. Corbett, Amanda H. Kashuba, Angela D. M. 2024-01-21T00:31:34Z 2024-01-21T00:31:34Z 2021-09-02 2007-10 0269-3879 https://pubs.kist.re.kr/handle/201004/134083 This work describes a sensitive method for the simultaneous determination of 17 antiretroviral drugs including nucleoside analog reverse transcriptase inhibitors, non-nucleoside analog reverse transcriptase inhibitors, protease inhibitors and a nucleotide analog reverse transcriptase inhibitor in 50 mu L of human plasma. This method employed high-performance liquid chromatography-tandem mass spectrometry with electrospray ionization. The analytes were monitored in multiple reaction monitoring mode and the polarity was switched from positive to negative to positive to detect all compounds after a single injection. A combination of liquid-liquid extraction and protein precipitation was used to extract all compounds, with at least 75% recovery for all analytes. Within- and between-day accuracies were at least 85% for 1-500 ng/mL for nelfinavir, indinavir and abacavir, 10-500 ng/mL for didanosine and stavudine and 5-500 ng/mL for all other compounds. This method is very effective for quantifying and screening antiretroviral drugs in clinical samples with limited (50 mu L) volumes. Copyright (c) 2007 John Wiley & Sons, Ltd. English WILEY REVERSE-TRANSCRIPTASE INHIBITORS HIV PROTEASE INHIBITORS BLOOD MONONUCLEAR-CELLS RAPID QUANTIFICATION NEVIRAPINE THERAPY INDINAVIR AMPRENAVIR SAQUINAVIR ATAZANAVIR Simultaneous determination of 17 antiretroviral drugs in human plasma for quantitative analysis with liquid chromatography-tandem mass spectrometry Article 10.1002/bmc.865 1 BIOMEDICAL CHROMATOGRAPHY, v.21, no.10, pp.1095 - 1104 BIOMEDICAL CHROMATOGRAPHY 21 10 1095 1104 scie scopus 000249846400014 2-s2.0-34548287060 Biochemical Research Methods Biochemistry & Molecular Biology Chemistry, Analytical Pharmacology & Pharmacy Biochemistry & Molecular Biology Chemistry Pharmacology & Pharmacy Article REVERSE-TRANSCRIPTASE INHIBITORS HIV PROTEASE INHIBITORS BLOOD MONONUCLEAR-CELLS RAPID QUANTIFICATION NEVIRAPINE THERAPY INDINAVIR AMPRENAVIR SAQUINAVIR ATAZANAVIR LC-MS-MS anti-retroviral drug plasma simultaneous determination