Hong, Jee Eun Pyo, Heesoo Park, Song-Ja Lee, Won 2024-01-21T00:32:14Z 2024-01-21T00:32:14Z 2021-09-02 2007-09-01 1570-0232 https://pubs.kist.re.kr/handle/201004/134113 This study examines a novel sample preparation method for the determination of 11 hydroxy metabolites of polychlorinated biphenyls (PCBs) in plasma and organ tissues, followed by gas chromatography with mass spectrometric detection (GC/MS). The clean-up method was optimized to eliminate the interference matter by using a silica column and 10 mL of n-hexane/dichloromethane (4:6, v/v) as an eluent. Solid-phase and solvent extraction procedures were used for the plasma and tissues samples, respectively. Compared to C-18 and C-8 solid-phase, C-2 showed higher extraction efficiency with n-hexane as the eluent for plasma. The hydroxy-PCB extraction recoveries achieved with this combined extraction and clean-up procedure from plasma ranged from 87 to 117%, while those from tissues ranged from 82 to 111%. The linear detector responses for propyl derivatives of hydroxy-PCBs were obtained with the coefficients of determination varying from 0.992 to 0.998 in the concentration range of 0. 1-20 ng mL(-1). The method detection limits ranged from 0. 1 to 0.5 ng niL(-1) 1mL of plasma and from 0. 1 to 0.5 ng g(-1) in 1g of tissues. This procedure was successfully applied to the study of 3-OH-2,3',4,4',5-PeCB in rat plasma and liver samples after intraperitoneal injection (20 mg/kg) of 2,3',4,4',5-PeCB. (C) 2007 Published by Elsevier B.V. English ELSEVIER SCIENCE BV METHYL SULFONE METABOLITES SOLID-PHASE EXTRACTION HUMAN-MILK LIQUID EXTRACTION MASS-SPECTROMETRY PCB METABOLITES DERIVATIZATION 2,4&apos ,5-TRICHLOROBIPHENYL IDENTIFICATION METHIONINE Determination of hydroxy metabolites of polychlorinated biphenyls in plasma and tissue by gas chromatography/mass spectrometry Article 10.1016/j.jchromb.2007.05.001 1 JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v.856, no.1-2, pp.1 - 8 JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 856 1-2 1 8 scie scopus 000249615100001 2-s2.0-34548163363 Biochemical Research Methods Chemistry, Analytical Biochemistry & Molecular Biology Chemistry Article METHYL SULFONE METABOLITES SOLID-PHASE EXTRACTION HUMAN-MILK LIQUID EXTRACTION MASS-SPECTROMETRY PCB METABOLITES DERIVATIZATION 2,4&apos ,5-TRICHLOROBIPHENYL IDENTIFICATION METHIONINE Hydroxy-PCBs GC/MS SPE clean-up plasma tissue