Babar, Sheikh Md. Enayetul Song, Eun Joo Hasan, Md. Nabiul Yoo, Young Sook 2024-01-21T00:32:34Z 2024-01-21T00:32:34Z 2022-01-10 2007-09 1615-9306 https://pubs.kist.re.kr/handle/201004/134129 To optimize the capillary electrophoretic separation conditions for leucine enkephalin (LE) and the immune complex of the LE and anti-LE reaction, an analysis using a three-level, three-factorial Box-Behnken design was performed. Three separation parameters, buffer pH (X-1), buffer concentration (X-2), and applied voltage (X-3), were chosen to observe the effect on separation responses. The responses were theoretical plate number, migration time of the LE peak, and resolution between the peaks. The optimum conditions and process validation were determined using statistical regression analysis and surface plot diagrams. The capillary electrophoresis optimum separation conditions were established to be 75 mM phosphate buffer at pH 7.00 with an applied separation voltage of 15 kV. By using the analysis technique, the prediction of responses was satisfactory and process verification yielded values within the +/- 5% range of the predicted efficiency. English WILEY-V C H VERLAG GMBH RESPONSE-SURFACE METHODOLOGY BOX-BEHNKEN DESIGN SYSTEMS BIOLOGY HYDROCHLORIDE VALIDATION Experimental design optimization of the capillary electrophoresis separation of leucine enkephalin and its immune complex Article 10.1002/jssc.200700145 1 JOURNAL OF SEPARATION SCIENCE, v.30, no.14, pp.2311 - 2319 JOURNAL OF SEPARATION SCIENCE 30 14 2311 2319 scie scopus 000249800300018 2-s2.0-34848845219 Chemistry, Analytical Chemistry Article RESPONSE-SURFACE METHODOLOGY BOX-BEHNKEN DESIGN SYSTEMS BIOLOGY HYDROCHLORIDE VALIDATION capillary electrophoresis Box-Behnken design leucine enkephalin