Son, Jung Hyun Yoo, Hye Hyun Kim, Dong-Hyun 2024-01-21T00:33:36Z 2024-01-21T00:33:36Z 2021-08-31 2007-09 1528-7394 https://pubs.kist.re.kr/handle/201004/134179 Sphingolipid metabolites in HL-60 cells were analyzed to gain an understanding of their roles in early events underlying hydrogen peroxide (H2O2)-induced apoptosis. Incubation of cells with H2O2 increased the intracellular levels of ceramides and sphinganine, but decreased those of ceramide 1-phosphates (ceramide 1-P) and sphingosine. The levels of sphingomyelins and sphingo- myelinase (SMase) activities were not affected by H2O2 treatment. These results were similar to the profiles induced by daunorubicin, an activator of serine palmitoyl CoA transferase (SPT), suggesting that H2O2 stimulated the de novo synthetic pathway of ceramides. L-cycloserine and fumonisin B, (FBI), specific inhibitors of de novo ceramide biosynthesis, suppressed the elevation of ceramides and sphinganine induced by 112021 which consequently reduced apoptotic cell death. Collectively, these results demonstrated that H2O2 increased the intracellular concentrations of ceramides via activation of a de novo biosynthetic pathway, and the enhanced ceramides might initiate apoptosis in HL-60 cells. English TAYLOR & FRANCIS INC STRESS-INDUCED APOPTOSIS SPHINGOSINE 1-PHOSPHATE OXIDATIVE STRESS SPHINGOMYELIN HYDROLYSIS ENDOTHELIAL-CELLS LLC-PK1 CELLS HL-60 CELLS GENERATION DEATH DIFFERENTIATION Activation of de novo synthetic pathway of ceramides is responsible for the initiation of hydrogen peroxide induced apoptosis in HI-60 cells Article 10.1080/15287390701434364 1 JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES, v.70, no.15-16, pp.1310 - 1318 JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH-PART A-CURRENT ISSUES 70 15-16 1310 1318 scie scopus 000248414300009 2-s2.0-34547156711 Environmental Sciences Public, Environmental & Occupational Health Toxicology Environmental Sciences & Ecology Public, Environmental & Occupational Health Toxicology Article STRESS-INDUCED APOPTOSIS SPHINGOSINE 1-PHOSPHATE OXIDATIVE STRESS SPHINGOMYELIN HYDROLYSIS ENDOTHELIAL-CELLS LLC-PK1 CELLS HL-60 CELLS GENERATION DEATH DIFFERENTIATION ceramide apoptosis analysis de novo synthesis