Park, Jung Sik Kim, Soyeon Han, Dong Keun Lee, Ji You Ghil, Sung Ho 2024-01-21T00:33:49Z 2024-01-21T00:33:49Z 2021-09-02 2007-08-31 1226-3613 https://pubs.kist.re.kr/handle/201004/134188 Skeletal muscle contains several precursor cells that generate muscle, bone, cartilage and blood cells. Although there are reports that skeletal muscle-derived cells can trans-differentiate into neural-lineage cells, methods for isolating precursor cells, and procedures for successful neural induction have not been fully established. Here, we show that the preplate cell isolation method, which separates cells based on their adhesion characteristics, permits separation of cells possessing neural precursor characteristics from other cells of skeletal muscle tissues. We term these isolated cells skeletal muscle-derived neural precursor cells (SMNPs). The isolated SMNPs constitutively expressed neural stem cell markers. In addition, we describe effective neural induction materials permitting the neuron-like cell differentiation of SMNPs. Treatment with retinoic acid or forskolin facilitated morphological changes in SMNPs; they differentiated into neuron-like cells that possessed specific neuronal markers. These results suggest that the preplate isolation method, and treatment with retinoic acid or forskolin, may provide vital assistance in the use of SMNPs in cell-based therapy of neuronal disease. English NATURE PUBLISHING GROUP CENTRAL-NERVOUS-SYSTEM STEM-CELLS NEURITE OUTGROWTH PROGENITOR CELLS ADIPOSE-TISSUE BONE-MARROW IN-VITRO REGENERATION GENERATION BRAIN Isolation of neural precursor cells from skeletal muscle tissues and their differentiation into neuron-like cells Article 10.1038/emm.2007.53 1 EXPERIMENTAL AND MOLECULAR MEDICINE, v.39, no.4, pp.483 - 490 EXPERIMENTAL AND MOLECULAR MEDICINE 39 4 483 490 scie scopus kci ART001077421 000249220200007 2-s2.0-34548842201 Biochemistry & Molecular Biology Medicine, Research & Experimental Biochemistry & Molecular Biology Research & Experimental Medicine Article CENTRAL-NERVOUS-SYSTEM STEM-CELLS NEURITE OUTGROWTH PROGENITOR CELLS ADIPOSE-TISSUE BONE-MARROW IN-VITRO REGENERATION GENERATION BRAIN cell differentiation muscle skeletal neuroD protein stem cells tissue therapy