Lee, Jin Hyung Hwang, Ee Taek Kim, Byoung Chan Lee, Sun-Mi Sang, Byoung-In Choi, Yong Su Kim, Jungbae Gu, Man Bock 2024-01-21T00:35:40Z 2024-01-21T00:35:40Z 2022-02-17 2007-07 0175-7598 https://pubs.kist.re.kr/handle/201004/134276 This study shows the preparation and application of enzyme-nanofiber composites for long-term stable operation. The enzyme-nanofiber composite was prepared by coating an enzyme aggregate, the esterase from Rhizopus oryzae, on the surface of the nanofibers. After immobilization on the nanofiber, the apparent K (m) for the immobilized esterase was 1.48-fold higher than that of the free esterase, with values of 0.98 and 1.35 mM for the free and immobilized enzymes, respectively. It was found that enzyme-nanofiber was very stable, even when the fibers were shaken in glass vials, preserving 80% of the initial activity for 100 days. In addition, the enzyme-nanofiber composite was used repeatedly in 30 cycles of substrate hydrolysis and still remained active. Consequently, the esterase-nanofiber composite was employed within a continuous reactor system to evaluate its use in a long-term and stable continuous substrate hydrolysis reaction. It was found that the production of p-nitrophenol was stable for at least 400 h. This study demonstrates that the enzyme-nanofiber composite can be used in both repeated-batch mode and a continuous mode for a long-term stable operation. English Springer Verlag Stable and continuous long-term enzymatic reaction using an enzyme-nanofiber composite Article 10.1007/s00253-007-0955-3 1 Applied Microbiology and Biotechnology, v.75, no.6, pp.1301 - 1307 Applied Microbiology and Biotechnology 75 6 1301 1307 N scopus 000247463100010 2-s2.0-34250888779 Biotechnology & Applied Microbiology Biotechnology & Applied Microbiology Article ULTRAFILTRATION MEMBRANE REACTOR IMPROVED STABILITY MESOPOROUS SILICA CROSS-LINKING NANOPARTICLES IMMOBILIZATION PERFORMANCE HYDROLYSIS CRYSTALS SYSTEM continuous reactor nanofiber esterase enzyme stabilization enzyme immobilization enzyme reactor