Song, Su Jin Jeon, Oju Yang, Hee Seok Han, Dong Keun Kim, Byung-Soo 2024-01-21T01:00:22Z 2024-01-21T01:00:22Z 2021-09-02 2007-07 1017-7825 https://pubs.kist.re.kr/handle/201004/134292 Human bone marrow-derived mesenchymal stern cells (hBMMSCs) must differentiate into osteogenic cells to allow for successful bone regeneration. In this study, we investigated the effects of different combinations of three soluble osteogenic differentiation-inducing factors [L-ascorbic acid (AC), beta-glycerophosphate (PG), and bone morphogenic protein-2 (BMP-2)] and the presence of a hydroxyapatite (HA) substrate on hBMMSC osteogenic differentiation in vitro. hBMMSCs were cultured in medium containing various combinations of the soluble factors on culture plates with or without HA coating. After 7 days of culture, alkaline phosphatase (ALP) activity, calcium deposition, and osteoprotegerin (OPG) and osteopontin (OPN) expression were measured. The effects of individual and combined factors were evaluated using a factorial analysis method. BMP-2 predominantly affected expression of early markers of osteogenic differentiation (ALP and OPG). HA had the highest positive effect on OPN expression and calcium deposition. The interaction between AC, beta G and HA had the second highest positive effect on ALP activity. English KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY MARROW STROMAL CELLS SIGNAL-REGULATED KINASE BONE-MARROW IN-VITRO ALKALINE-PHOSPHATASE COMPOSITE SCAFFOLDS OSTEOBLAST PHENOTYPE PROLIFERATION PLASMA GROWTH Effects of culture conditions on osteogenic differentiation in human mesenchymal stem cells Article 1 JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.17, no.7, pp.1113 - 1119 JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY 17 7 1113 1119 scie scopus kci 000248379500008 2-s2.0-34547979075 Biotechnology & Applied Microbiology Microbiology Biotechnology & Applied Microbiology Microbiology Article MARROW STROMAL CELLS SIGNAL-REGULATED KINASE BONE-MARROW IN-VITRO ALKALINE-PHOSPHATASE COMPOSITE SCAFFOLDS OSTEOBLAST PHENOTYPE PROLIFERATION PLASMA GROWTH bone differentiation mesenchymal stem cell