Vukoti, Krishna Moorthy Lee, Won-Kyu Kim, Ho-Jun Kim, Ick Young Yang, Eun Gyeong Lee, Cheolju Yu, Yeon Gyu 2024-01-21T01:00:57Z 2024-01-21T01:00:57Z 2021-08-31 2007-06-20 0253-2964 https://pubs.kist.re.kr/handle/201004/134319 Leukotriene B4 (LTB4) is a potent chemoattractant for leukocytes and considered to be an inflammatory mediator. Human BLT2 (hBLT2) is a low-affinity G-protein coupled receptor for LTB4 and mediates pertussis toxin-sensitive chemotactic cell movement. Here, we dissected the interaction between hBLT2 and G-protein alpha subunits using GST fusion proteins containing intracellular regions of hBLT2 and various G alpha protein including G alpha i1, G alpha i2, G alpha i3, G alpha s1, G alpha o1, and G alpha z. Among the tested G alpha subunits, G alpha i3 showed the highest binding to the third intracellular loop region of hBLT2 with a dissociation constant (K-D) of 5.0 x 10(-6) M. These results suggest that G alpha i3 has the highest affinity to hBLT2, and the third intracellular loop region of hBLT2 is the major component for the interaction with G alpha i3. English WILEY-V C H VERLAG GMBH LEUKOTRIENE B-4 RECEPTOR COLLAGEN-INDUCED ARTHRITIS LIGHT-ACTIVATED RHODOPSIN ANTAGONIST LY293111 DEFICIENT MICE IN-VITRO BLT2 SITE MUTAGENESIS BINDING Molecular dissection of the interaction between hBLT2 and the G protein alpha subunits Article 1 BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.28, no.6, pp.1005 - 1009 BULLETIN OF THE KOREAN CHEMICAL SOCIETY 28 6 1005 1009 scie scopus kci ART001064061 000248208700021 2-s2.0-34250876289 Chemistry, Multidisciplinary Chemistry Article LEUKOTRIENE B-4 RECEPTOR COLLAGEN-INDUCED ARTHRITIS LIGHT-ACTIVATED RHODOPSIN ANTAGONIST LY293111 DEFICIENT MICE IN-VITRO BLT2 SITE MUTAGENESIS BINDING leukotriene B4 hBLT2 G-protein intracellular loop GPCR-G protein interaction