Lee, Soo Jae Kim, Kyun-Hwan Park, Ji Sook Jung, Jin Woo Kim, Young Hwan Kim, Sang Kyung Kim, Wan-Seok Goh, Hyun-gyung Kim, Soo-hyun Yoo, Jung-Sun Kim, Dong-Wook Kim, Kwang Pyo 2024-01-21T01:01:03Z 2024-01-21T01:01:03Z 2021-09-02 2007-06-08 0006-291X https://pubs.kist.re.kr/handle/201004/134324 This study was designed to identify the cell surface protein markers that can differentiate between chronic myeloid leukemia (CML) and acute promyelocytic leukemia cells (APL). The differentially expressed plasma membrane proteins were analyzed between CML cell line (K562) and APL cell line (NB4) using the comparative proteomic approach. The cell membrane proteins were enriched by labeling with a membrane-impermeable biotinylation reagent, sulfo-NHS-SS-Biotin, and subjected to liquid chromatography tandem mass spectrometry (LC-MS/MS). By comparative proteomic analysis of K562 and NB4 cells, we identified 25 membrane and 14 membrane-associated proteins. The result of LC-MS/MS combined with chemical tagging method was validated by confirming the expression and localization of one of the differentially expressed plasma membrane proteins, CD43, by FACS and confocal microscopy. Our results indicate that CD43 could be a potential candidate for differentiating CML from APL. (c) 2007 Elsevier Inc. All rights reserved. English ACADEMIC PRESS INC ELSEVIER SCIENCE CD43 IDENTIFICATION TRANSPORT PROTEOME Comparative analysis of cell surface proteins in chronic and acute leukemia cell lines Article 10.1016/j.bbrc.2007.03.191 1 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.357, no.3, pp.620 - 626 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 357 3 620 626 scie scopus 000246382700009 2-s2.0-34247478903 Biochemistry & Molecular Biology Biophysics Biochemistry & Molecular Biology Biophysics Article CD43 IDENTIFICATION TRANSPORT PROTEOME proteomics chronic myeloid leukemia acute promyelocytic leukemia mass spectrometry