Nam, Ki-Hyun Kwon, Oh Yeun Sugiyama, Kanako Lee, Won-Ho Kim, Young Kwan Song, Hyun Kyu Kim, Eunice Eunkyung Park, Sam-Yong Jeon, Hyesung Hwang, Kwang Yeon 2024-01-21T01:06:15Z 2024-01-21T01:06:15Z 2021-09-05 2007-03-23 0006-291X https://pubs.kist.re.kr/handle/201004/134532 The photoswitching behavior of green fluorescent proteins (GFPs) or GFP-like proteins is increasingly recognized as a new technique for optical marking. Recently, Ando and his colleagues developed a new green fluorescent protein Dronpa, which possesses the unique photochromic property of being photoswitchable in a non-destructive manner. To better understand this mechanism, we determined the crystal structures of a new GFP Dronpa and its mutant C62S, at 1.9 A and 1.8 A, respectively. Determination of the structures demonstrates that a unique hydrogen-bonding network and the sulfur atom of the chromophore are critical to the photoswitching property of Dronpa. Reversible photoswitching was lost in cells expressing the Dronpa-C62S upon repetitive irradiation compared to the native protein. Structural and mutational analyses reveal the chemical basis for the functional properties of photoswitchable fluorescent proteins and provide the basis for subsequent coherent engineering of this subfamily of Dronpa homolog's. (c) 2007 Elsevier Inc. All rights reserved. English ACADEMIC PRESS INC ELSEVIER SCIENCE CRYSTAL-STRUCTURE Structural characterization of the photoswitchable fluorescent protein Dronpa-C62S Article 10.1016/j.bbrc.2007.01.086 1 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.354, no.4, pp.962 - 967 BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 354 4 962 967 scie scopus 000244411800020 2-s2.0-33846786902 Biochemistry & Molecular Biology Biophysics Biochemistry & Molecular Biology Biophysics Article CRYSTAL-STRUCTURE Dronpa real-time imaging photoswitch able Dronpa-C62S isomerization