Jin, Cheng Zhe Park, So Ra Choi, Byung Hyune Park, Kwideok Min, Byoung-Hyun 2024-01-21T01:31:00Z 2024-01-21T01:31:00Z 2021-09-05 2007-03 0160-564X https://pubs.kist.re.kr/handle/201004/134583 We have observed in our previous study that a cell-derived extracellular matrix (ECM) scaffold could assure the growth of a cartilage tissue construct in vitro. The purpose of the present study was to evaluate the feasibility of a chondrocyte-seeded cell-derived ECM scaffold by implanting it in vivo in nude mouse. A porous cell-derived ECM scaffold was prepared with a freeze-drying protocol using porcine chondrocytes. Rabbit articular chondrocytes were seeded onto the scaffold and cultured for 2 days in vitro, and then implanted into the nude mouse subcutaneously. They were retrieved at 1, 2, and 3 weeks postimplantation. Under macroscopic analysis, the cartilage-like tissue formation matured with time and developed a smooth, white surface. Contrary to the control (in which no cells were seeded), the size of the neocartilage tissue increased slightly by the third week and remained more stable. Total glycosaminoglycan (GAG) content and the GAG/DNA ratio increased significantly with time in the chemical analysis. The histology exhibited a sustained accumulation of newly synthesized sulfated proteoglycans. Immunohistochemistry, Western blot, and reverse transcriptase-polymerase chain reaction (RT-PCR) clearly identified type II collagen at all time points. Compressive strength of in vivo neocartilage increased from 0.45 +/- 0.06 MPa at 1 week to 1.18 +/- 0.17 MPa at 3 weeks. In conclusion, this study demonstrated that the cell-derived ECM scaffold could provide chondrocytes with favorable in vivo environment to produce a hyaline-like cartilage tissue. English WILEY INTRINSIC MECHANICAL-PROPERTIES CHONDROCYTES BEHAVIOR REPAIR GEL In vivo cartilage tissue engineering using a cell-derived extracellular matrix scaffold Article 10.1111/j.1525-1594.2007.00363.x 1 ARTIFICIAL ORGANS, v.31, no.3, pp.183 - 192 ARTIFICIAL ORGANS 31 3 183 192 scie scopus 000244612100003 2-s2.0-33847664781 Engineering, Biomedical Transplantation Engineering Transplantation Article INTRINSIC MECHANICAL-PROPERTIES CHONDROCYTES BEHAVIOR REPAIR GEL cell-derived extracellular matrix scaffold nude mouse tissue-engineered cartilage chondrocytes