Kim, Sang-Heon Akaike, Toshihiro 2024-01-21T01:31:17Z 2024-01-21T01:31:17Z 2021-09-05 2007-03 1076-3279 https://pubs.kist.re.kr/handle/201004/134595 Understanding hepatocellular signaling occurring in biomaterial systems is important for successful hepatic tissue engineering. Toward this end, we employed synthetic glycopolymers, as artificial matrices, to examine integrin-mediated epidermal growth factor (EGF) signaling in primary hepatocyte cultures. We dispersed hepatocytes on a collagen matrix or on a synthetic glycopolymer matrix and subsequently stimulated them with EGF. Only hepatocytes cultured on collagen proliferated, and we observed significant expression of cyclin B1 in these cells. Pharmacological agents, LY294004 (a phosphatidylinositol [PI] 3-kinase inhibitor) and AG1478 (an EGF kinase receptor inhibitor), blocked hepatocyte proliferation and cyclin B1 expression. In addition, EGF-stimulated hepatocytes formed spheroids, exhibited membrane ruffling, and increased tryptophan 2,3-oxygenase (TO) expression when cultured on glycopolymer matrices. Interestingly, PI 3-kinase inhibition suppressed membrane ruffling, spheroid formation, and TO expression. Taken together, this data suggests PI 3-kinase plays an important role in mediating cross talk between integrin and the EGF signaling pathways in primary hepatocyte cultures. English MARY ANN LIEBERT INC SYNTHETIC GLYCOPOLYMER MATRIX RAT HEPATOCYTES EXTRACELLULAR-MATRIX ASIALOGLYCOPROTEIN RECEPTOR PARTIAL-HEPATECTOMY GENE TRANSFECTION ADHESION LIVER TRANSDUCTION EFFICIENCY Epidermal growth factor signaling for matrix-dependent cell proliferation and differentiation in primary cultured hepatocytes Article 10.1089/ten.2006.0104 1 TISSUE ENGINEERING, v.13, no.3, pp.601 - 609 TISSUE ENGINEERING 13 3 601 609 scie scopus 000245464500015 2-s2.0-34147206028 Cell & Tissue Engineering Cell Biology Article SYNTHETIC GLYCOPOLYMER MATRIX RAT HEPATOCYTES EXTRACELLULAR-MATRIX ASIALOGLYCOPROTEIN RECEPTOR PARTIAL-HEPATECTOMY GENE TRANSFECTION ADHESION LIVER TRANSDUCTION EFFICIENCY EGF Integrin glycopolymer cell adhesion liver tissue engineering cell signaling