Kang, Min-Jung Hwang, Yong Hee Lee, Won Kim, Dong-Hyun 2024-01-21T01:35:27Z 2024-01-21T01:35:27Z 2021-09-05 2007-01 0951-4198 https://pubs.kist.re.kr/handle/201004/134768 Electrospray ionization (ESI) mass spectra of 15 anti-estrogenic substances, beta 2-agonists and mesocarb were investigated in terms of fragmentation patterns. On the basis of this product ion information, a simultaneous screening method for anti-estrogenic substances, beta 2-agonists and mesocarb was developed for doping control purposes. After hydrolysis, liquid-liquid extraction was adopted for the sample preparation. The recoveries for all compounds were 30 and 96%. A single liquid chromatography/tandem mass spectrometry (LC/MS/MS) analysis could be performed in 13 min for the analysis of 15 anti-estrogenic substances, beta 2-agonists and mesocarb. A quantitative analysis was also validated. Inaccuracies were below +/- 12% and precisions varied from 0 to 15.8%. The limit of detection was below 10 ng/mL except formestane (300 ng/mL) and aminoglutethimide (100 ng/mL). The validated method was applied for the analysis of excretion samples. Copyright (c) 2006 John Wiley & Sons, Ltd. English John Wiley & Sons Inc. Validation and application of a screening method for beta 2-agonists, anti-estrogenic substances and mesocarb in human urine using liquid chromatography/tandem mass spectrometry Article 10.1002/rcm.2834 1 Rapid Communications in Mass Spectrometry, v.21, no.2, pp.252 - 264 Rapid Communications in Mass Spectrometry 21 2 252 264 N scie scopus 000243473500021 2-s2.0-33846204287 Biochemical Research Methods Chemistry, Analytical Spectroscopy Biochemistry & Molecular Biology Chemistry Spectroscopy Article AROMATASE INHIBITOR QUANTITATIVE-ANALYSIS BETA-AGONISTS IDENTIFICATION CLENBUTEROL EXEMESTANE EXTRACTION CIMATEROL PLASMA ONLINE screening method anti-estrogenic substances b2-agonists mesorcarb LC-MS