Chun, Sung-Min Kim, Yoo-Li Choi, Hee Baeg Oh, Yong-Taek Kim, Yoo-Jin Lee, Seok Kim, Tai-Gyu Yang, Eun Gyeong Park, Yong-Keun Kim, Dong-Wook Han, Byoung-Don 2024-01-21T01:35:55Z 2024-01-21T01:35:55Z 2021-09-04 2007-01 1177-1062 https://pubs.kist.re.kr/handle/201004/134788 Background: Identification of specific chromosomal translocations is essential for the diagnosis and prognosis of leukemia. In this study, we employ DNA microarray technology to detect chromosomal aberrations in patients with chronic myeloid leukemia (CML) and acute myeloid leukemia (AML), as well as in leukemic cell lines. Methods: Reverse transcription using a random 9-mer primer was performed with total RNA from patients and leukemic cells lines. Multiplex PCR reactions using four groups of primer sets were then performed for amplification of cDNA from reverse-transcribed total RNA samples. Normal and fusion sequences were distinguished by hybridization of the amplified cDNA to a selective oligonucleotide array (SOA) containing 20-30mer synthetic probes. A total of 23 sets of oligomers; were fabricated on glass slides for the detection of normal and fusion genes, as follows: BCR/ABL, AML/EAP, AML/ETO, AML/MDS, PML/RARA, NUMA1/RARA, PLZF/RARA, and CBFB/MYH. Results: Gene translocation in leukemia was effectively identified with the SOA containing various leukemia-specific fusion and normal control sequences. Leukemic fusion sequences from patients and cell lines hybridized specifically to their complementary probes. The probe sets differing by approximate to 50% at their 5' or 3' ends could distinguish between normal and fusion sequences. The entire process of detection was completed within 8 hours using the SOA method. Conclusions: Probe sets on SOA can effectively discriminate between leukemia-specific fusion and normal sequences with a chip hybridization procedure. The oligonucleotide array presents several advantages in identifying leukemic gene translocations, such as multiplex screening, relatively low cost, and speed. English ADIS INTERNATIONAL LTD POLYMERASE-CHAIN-REACTION ACUTE LYMPHOBLASTIC-LEUKEMIA ACUTE MYELOID-LEUKEMIA REVERSE-TRANSCRIPTION RT-PCR RESIDUAL DISEASE TRANSLOCATIONS BCR MICROARRAYS DIAGNOSIS Identification of leukemia-specific fusion gene transcripts with a novel oligonucleotide array Article 10.1007/BF03256220 1 MOLECULAR DIAGNOSIS & THERAPY, v.11, no.1, pp.21 - 28 MOLECULAR DIAGNOSIS & THERAPY 11 1 21 28 scie scopus 000245013300003 2-s2.0-33846983212 Genetics & Heredity Pharmacology & Pharmacy Genetics & Heredity Pharmacology & Pharmacy Article POLYMERASE-CHAIN-REACTION ACUTE LYMPHOBLASTIC-LEUKEMIA ACUTE MYELOID-LEUKEMIA REVERSE-TRANSCRIPTION RT-PCR RESIDUAL DISEASE TRANSLOCATIONS BCR MICROARRAYS DIAGNOSIS