Jeon, Byoung Seung 이선미 김용환 구만복 채희정 상병인 2024-01-21T02:00:57Z 2024-01-21T02:00:57Z 2021-09-06 2006-12 1738-7264 https://pubs.kist.re.kr/handle/201004/134848 Two mesophilic trickling bed bioreactors filled with two different types of media, hydrophilic- and hydrophobic-cubes, were designed and conducted for hydrogen production under the anaerobic fermentation of sucrose. Each bioreactor consisted of the column packed with polymeric cubes and inoculated with heat-treated sludge obtained from anaerobic digestion tank. A defined medium containing sucrose was fed by the different hydraulic retention time(HRT), and recycle rate. Hydrogen concentrations in gas-phase were constant, averaging 40% of biogas throughout the operation. Hydrogen production rate was increased till 10.5 Lh-1 L-1 of bioreactor when influent sucrose concentrations and recycle rates were varied. At the same time, the hydrogen production rate with hydrophobic media application was higher than its hydrophilic media application. No methane was detected when the reactor was under a normal operation. The major fermentation by-products in the liquid effluent of the both trickling biofilters were acetate, butyrate and lactate. In order to run in the long term operation of both reactor filled with hydrophilic and hydrophobic media, biofilm accumulation on hydrophilic media and biogas produced should be controlled through some process such as periodical backwashing or gas-purging. Four sample were collected from each reactor on the opposite hydrogen production rate, and their bacterial communities were compared by terminal restriction fragment length polymorphism (T-RFLP) analysis of PCR products generated using bacterial 16s rRNA gene primers (8f and 926r). It was expressed a marked difference in bacterial communities of both reactors. The trickling bed bioreactor with hydrophobic media demonstrates the feasibility of the process to produce hydrogen gas. A likely application of this reactor technology can be hydrogen gas recovery from pre-treatment of high carbohydrate-containing wastewaters. Korean 한국수소및신에너지학회 소수성 및 친수성 담체를 이용한 생물학적 수소생산 The Fermentative Hydrogen Production in Trickling Bed Biofilter Filled with Hydrophilic-and Hydrophobic-Media Article 2 한국수소및신에너지학회논문집, v.17, no.4, pp.379 - 388 한국수소및신에너지학회논문집 17 4 379 388 N kci ART001166786 hydrophilic hydrophobic T-RFLP HRT substrate concentration hydrophilic hydrophobic T-RFLP HRT substrate concentration 친수성 소수성 제한효소 다형법 수리학적 체류시간 기질 농도