Cho, Eun Ju Lee, Young A. Yoo, Hye Hyun Yokozawa, Takako 2024-01-21T02:03:10Z 2024-01-21T02:03:10Z 2021-08-31 2006-12 0301-4800 https://pubs.kist.re.kr/handle/201004/134943 The antioxidative effect and protective potential against diabetes of the broccoli flower were investigated both in vitro and in a diabetic rat model. Among fractions of MeOH, CH2Cl2, BuOH, and H2O, the BuOH fraction exerted the strongest inhibitory activities on 1,1-diphenyl-2-picrylhydrazyl radical, radical-induced protein oxidation, and nitric oxide generation by sodium nitroprusside. The in vitro results suggest that the BuOH fraction from the broccoli flower has a protective potential against oxidative stress. The rat model with diabetes induced by streptozotocin was employed to evaluate the protective effect of the BuOH fraction in vivo. Diabetic rats showed reduced body weight gain and heavier kidney and liver weights than normal rats, while oral administration of the BuOH fraction at,an oral dose of 100 or 200 mg/kg body weight/d for 20 d attenuated the physiological changes induced by diabetes. In addition, oral administration of the BuOH fraction to diabetic rats led to significant decreases in serum glucose and glycosylated protein, while it resulted in the increase of serum albumin, implying that the BuOH fraction improves the abnormal metabolism of glucose and protein that leads to oxidative stress. Moreover, it significantly reduced thiobarbituric acid-reactive substance levels in serum, hepatic and renal mitochondria. This suggests that the BuOH fraction would alleviate the oxidative stress associated with diabetes through the inhibition of lipid peroxidation. The present study demonstrates that the BuOH fraction has an antioxidative effect in vitro and it protects against oxidative stress induced by diabetes in an in vivo model. English CENTER ACADEMIC PUBL JAPAN ANTIOXIDANT TREATMENT LIPID-PEROXIDATION STRESS VEGETABLES MECHANISM PATHOLOGY TOXICITY STROKE FRUIT CELLS Protective effects of broccoli (Brassica oleracea) against oxidative damage in vitro and in vivo Article 10.3177/jnsv.52.437 1 JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY, v.52, no.6, pp.437 - 444 JOURNAL OF NUTRITIONAL SCIENCE AND VITAMINOLOGY 52 6 437 444 scie scopus 000243208000007 Nutrition & Dietetics Nutrition & Dietetics Article ANTIOXIDANT TREATMENT LIPID-PEROXIDATION STRESS VEGETABLES MECHANISM PATHOLOGY TOXICITY STROKE FRUIT CELLS broccoli antioxidative activity oxidative stress diabetes streptozotocin