Yoo, Hye Hyun Son, Junghyun Kim, Dong-Hyun 2024-01-21T02:03:38Z 2024-01-21T02:03:38Z 2021-09-01 2006-11-07 1570-0232 https://pubs.kist.re.kr/handle/201004/134962 A liquid chromatography-electrospray ionization tandem mass spectrometric (LC-MS/MS) method was developed for the simultaneous qualitative and quantitative determination of sphingolipid metabolites such as ceramides, sphingisine, sphinganine, sphingomyelins, and ceramide 1-phosphates in the extracts of human promyelocytic leukemia cells (HL-60). The assay uses C-4 ceramide as an internal standard; simple liquid extraction; a short XTerra MS C-18 (3 mu m, 50 mm x 2.0 mm) column; a gradient mobile phase of 5 mM ammonium formate (pH 4.0)/methanol/tetrahydrofuran (5/2/3 -> 1/2/7); mass spectrometric detection using electrospray ionization. This LC-MS/MS method allowed the identification of 22 sphingolipid derivatives at pmol levels. In addition, this technique was successfully applied to analyze the changes of the sphingolipids profiles in cancer cells treated with apoptosis inducing agents, C-2 ceramide and H2O2. (c) 2006 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE BV QUANTITATIVE-DETERMINATION METHOD FREE SPHINGOID BASES INDUCED APOPTOSIS HL-60 CELLS DIFFERENTIATION QUANTIFICATION SPHINGANINE RANGE Liquid chromatography-tandem mass spectrometric determination of ceramides and related lipid species in cellular extracts Article 10.1016/j.jchromb.2006.06.025 1 JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, v.843, no.2, pp.327 - 333 JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 843 2 327 333 scie scopus 000242361400027 2-s2.0-33751018567 Biochemical Research Methods Chemistry, Analytical Biochemistry & Molecular Biology Chemistry Article QUANTITATIVE-DETERMINATION METHOD FREE SPHINGOID BASES INDUCED APOPTOSIS HL-60 CELLS DIFFERENTIATION QUANTIFICATION SPHINGANINE RANGE sphingolipid ceramides LC-MS/MS HL-60 cells