Kim, Tae Woo Chung, Hesson Kwon, Ick Chan Sung, Ha Chin Kang, Tae Heung Han, Hee Dong Jeong, Seo Young 2024-01-21T02:04:58Z 2024-01-21T02:04:58Z 2021-09-01 2006-10-31 1016-8478 https://pubs.kist.re.kr/handle/201004/135015 Delivery of DNA vaccines to airway mucosa would be an ideal method for mucosal immunization. However, there have been few reports of a suitable gene delivery system. In this study we used a cationic emulsion to immunize mice via the intranasal route with pCMV-S coding for Hepatitis B virus surface antigen (HBsAg). Complexing pCMV-S with a cationic emulsion dramatically enhanced HBsAg expression in both nasal tissue and lung, and was associated with increases in the levels of HBs-specific Abs in serum and mucosal fluids, of cytotoxic T lymphocytes (CTL) in the spleen and cervical and iliac lymph nodes, and of delayed-type hypersensitivity (DTH) against HBsAg. In contrast, very weak humoral and cellular immunities were observed following immunization with naked DNA. In support of these observations, a higher proliferative response of spleenocytes was detected in the group immunized with the emulsion/pCMV-S complex than in the group immunized with naked pCMV-S. These findings may facilitate development of an emulsion-mediated gene vaccination technique for use against intracellular pathogens that invade mucosal surfaces. English SPRINGER SINGAPORE PTE LTD VACCINES IMMUNIZATION DELIVERY SYSTEM SARS Induction of immunity against Hepatitis B virus surface antigen by intranasal DNA vaccination using a cationic emulsion as a mucosal gene carrier Article 1 MOLECULES AND CELLS, v.22, no.2, pp.175 - 181 MOLECULES AND CELLS 22 2 175 181 scie scopus kci ART001028528 000241798100008 2-s2.0-33847650537 Biochemistry & Molecular Biology Cell Biology Biochemistry & Molecular Biology Cell Biology Article VACCINES IMMUNIZATION DELIVERY SYSTEM SARS cationic lipid emulsion Hepatitis B virus mucosal DNA vaccine