Ahn, Hak Jun Koketsu, Mamoru Yang, Eun Mi Kim, Yong Man Ishihara, Hideharu Yang, Hyun Ok 2024-01-21T02:05:12Z 2024-01-21T02:05:12Z 2021-09-01 2006-10-15 0730-2312 https://pubs.kist.re.kr/handle/201004/135025 We examined the ability of the synthetic selenium compound, 2-(4-methylphenyl)-1,3-selenazol-4-one (hereafter designated 3a), to induce apoptosis in a human ovarian cancer cell line (SKOV3) and a human leukemia cell line (HL-60). Flow cytometry showed that 3a treatment induced apoptosis in both cell lines to degrees comparable to that of the positive control, paclitaxel. Apoptosis was measured by PS externalization, DNA fragmentation and decreased mitochondrial membrane potential (MMP). However, analysis of the mechanism of action revealed differences between the responses of the two cell lines. Treatment with 3a arrested the cell cycle and induced caspase-3 activation in HL-60 cells, but not in SKOV3 cells. In contrast, 3a treatment induced apoptosis through translocation of AIF, a novel proapoptotic protein, in SKOV3 cells, but not in HL-60 cells. Collectively, our data demonstrated that 3a induced apoptosis in both cell lines, but via different action mechanisms. English WILEY-LISS TAXOL-INDUCED APOPTOSIS CYTOCHROME-C CARCINOMA CELLS SELENIUM ACTIVATION DEATH OLIGOMERIZATION PROCASPASE-9 INHIBITION INDUCTION 2-(4-methylphenyl)-1,3-selenazol-4-one induces apoptosis by different mechanisms in SKOV3 and HL 60 cells Article 10.1002/jcb.20973 1 JOURNAL OF CELLULAR BIOCHEMISTRY, v.99, no.3, pp.807 - 815 JOURNAL OF CELLULAR BIOCHEMISTRY 99 3 807 815 scie scopus 000240787800013 2-s2.0-33749474317 Biochemistry & Molecular Biology Cell Biology Biochemistry & Molecular Biology Cell Biology Article TAXOL-INDUCED APOPTOSIS CYTOCHROME-C CARCINOMA CELLS SELENIUM ACTIVATION DEATH OLIGOMERIZATION PROCASPASE-9 INHIBITION INDUCTION selenium apoptosis AIF caspase mitochondria