Kim, Deok-Ho Park, Jungyul Suh, Kahp Y. Kim, Pilnam Choi, Seung Kyu Ryu, Seokchang Park, Sukho Lee, Sang Ho Kim, Byungkyu 2024-01-21T02:05:19Z 2024-01-21T02:05:19Z 2021-09-01 2006-10-12 0925-4005 https://pubs.kist.re.kr/handle/201004/135030 In this paper, we report a microfabrication approach to patterned three-dimensional growth of muscle cells for powering biohybrid microdevices. The microwells confined by adhesion-resistant polyethylene glycol (PEG) microstructures were patterned on SiO2-based substrate with controllable surface topography for muscle cell culturing. The morphology and motility of neonatal rat cardiac myocytes patterned within microwells were analyzed with different topographical heights of the PEG barrier. It was found that isolated aggregates of cardiac muscles showed various beating activities depending on the morphology and the number of cells. Furthermore, three-dimensionally grown cardiac muscle cells mediated by a higher physical barrier of PEG microstructures generated higher contraction force with faster beating frequency than those of cells attached to the collagen-coated surface on the culture dish (control), suggesting that control over surface topography of microwells for cell growth would be potentially useful in engineering cell motors. Thus the technique presented here would provide a valuable platform for optimizing the activity and functions of patterned muscle cells in building up integrated bioactuated microdevices. (c) 2005 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE SA CELL-ADHESION FORCE PROTEIN PDMS Fabrication of patterned micromuscles with high activity for powering biohybrid microdevices Article 10.1016/j.snb.2005.11.051 1 SENSORS AND ACTUATORS B-CHEMICAL, v.117, no.2, pp.391 - 400 SENSORS AND ACTUATORS B-CHEMICAL 117 2 391 400 scie scopus 000239876900011 2-s2.0-33748180652 Chemistry, Analytical Electrochemistry Instruments & Instrumentation Chemistry Electrochemistry Instruments & Instrumentation Article; Proceedings Paper CELL-ADHESION FORCE PROTEIN PDMS micropatterning cardiac muscles polyethylene glycol (PEG) capillary lithography bioactuator biohybrid microdevice