Cho, Sung-Hee
Jung, Byung Hwa
Lee, Won-Yong
Chung, Bong Chul
2024-01-21T02:06:29Z
2024-01-21T02:06:29Z
2021-09-01
2006-10
0269-3879
https://pubs.kist.re.kr/handle/201004/135078
A simple and highly sensitive method for the quantification of dehydroepiandrosterone-3-sulfate (DHEAS) in human plasma was developed. DBEAS was directly determined in plasma using column-switching liquid chromatography/mass spectrometry (LC-MS). The plasma was filtered with a membrane filter. The filtrate was injected onto a pre-column without further sample preparation such as extraction or derivatization. The pre-column was washed with an aqueous solution to remove interference and the analyte was eluted into a reversed-phase C, analytical column for separation and detection using a column-switching valve. The calibration range of DHEAS was 0.01-10 mu mol/L, and the linearity of the method was 0.999. The limit of detection (LOD) at a signal-to-noise (S/N) ratio of 3 was 5 nmol/L. The accuracy and precision (%CV) were less than 10% in within-day and day-to-day variations. To explore the relationship between Alzheimer's disease and the DHEAS level in human plasma, the concentrations of DHEAS in female patients with Alzheimer's disease (n = 20) and in normal female subjects (n = 20) were measured. The level of DHEAS was significantly decreased in the plasma of patients with Alzheimer's disease (p < 0.0002) compared with that in normal subjects. From the results, we concluded that our method is sufficiently sensitivity and reliability for the quantification of DHEAS in clinical samples. Plasma DHEAS concentration could be an important marker to understand the pathogenesis of Alzheimer's disease. Copyright (c) 2006 John Wiley & Sons, Ltd.
English
WILEY
IONIZATION-MASS-SPECTROMETRY
DEHYDROEPIANDROSTERONE-SULFATE
CARDIOVASCULAR-DISEASE
ATHEROSCLEROSIS
ANDROGENS
MORTALITY
DEMENTIA
WOMEN
RISK
Rapid column-switching liquid chromatography/mass spectrometric assay for DHEA-sulfate in the plasma of patients with Alzheimer's disease
Article
10.1002/bmc.647
1
BIOMEDICAL CHROMATOGRAPHY, v.20, no.10, pp.1093 - 1097
BIOMEDICAL CHROMATOGRAPHY
20
10
1093
1097
scie
scopus
000242198100019
2-s2.0-33750686950
Biochemical Research Methods
Biochemistry & Molecular Biology
Chemistry, Analytical
Pharmacology & Pharmacy
Biochemistry & Molecular Biology
Chemistry
Pharmacology & Pharmacy
Article
IONIZATION-MASS-SPECTROMETRY
DEHYDROEPIANDROSTERONE-SULFATE
CARDIOVASCULAR-DISEASE
ATHEROSCLEROSIS
ANDROGENS
MORTALITY
DEMENTIA
WOMEN
RISK
dehydroepiandrosterone-3-sulfate
column-switching liquid chromatography
mass spectrometry
plasma
Alzheimer&apos
s disease