Ryu, Hye Kyung Jung, Byung Hwa Kim, Kyung Mee Yoo, Eun Ah Woo, Jeong-Taek Chung, Bong Chul 2024-01-21T02:06:30Z 2024-01-21T02:06:30Z 2021-09-01 2006-10 0269-3879 https://pubs.kist.re.kr/handle/201004/135079 This work describes a sensitive method for determining cholesterol in human hair using GC-MS. In this study, we used a very small amount of hair, only 1 mg, to quantify cholesterol. We also can achieve more effective purification and a good recovery over 92% with solid-phase extraction using an Oasis HLB cartridge. The intra-day and inter-day precision and accuracy values were less than 7.08%. Cholesterol was determined to be in the range of 355-1693 mu g/g in healthy human hair. We tested the concentration correlation between the serum and hair to examine the feasibility of using the hair cholesterol level as all index of the serum cholesterol level. The correlation between the serum cholesterol was 0.86 (r-value) in patients with hypercholesterolemia. This finding indicates that, in the clinical field, hair could replace serum in cholesterol level measurement. Copyright (c) 2006 John Wiley & Sons, Ltd. English WILEY INTEGRAL LIPIDS DISEASE ALZHEIMER PLASMA AGE Determination of cholesterol in human hair using gas chromatography-mass spectrometry Article 10.1002/bmc.615 1 BIOMEDICAL CHROMATOGRAPHY, v.20, no.10, pp.999 - 1003 BIOMEDICAL CHROMATOGRAPHY 20 10 999 1003 scie scopus 000242198100004 2-s2.0-33750728031 Biochemical Research Methods Biochemistry & Molecular Biology Chemistry, Analytical Pharmacology & Pharmacy Biochemistry & Molecular Biology Chemistry Pharmacology & Pharmacy Article INTEGRAL LIPIDS DISEASE ALZHEIMER PLASMA AGE cholesterol hypercholesterolemia hair serum stable isotope dilution gas chromatography-mass spectrometry