Kim, Youn-Jung Kim, Mi-Soon Jeon, Hee-Kyoung Ryu, Jae-Chun 2024-01-21T02:30:32Z 2024-01-21T02:30:32Z 2021-09-01 2006-09-30 1738-642X https://pubs.kist.re.kr/handle/201004/135098 Khal was a synthetic congener of halocidin, a heterodimeric peptide consisting of 19 and 15 amino acid residues detected in Halocynthia aurantium. This compound was considered a candidate for the development of a novel peptide antibiotic. The genotoxicity of Khal was subjected to high throughput toxicity screening (HTTS) because they revealed strong antibacterial effects. Mouse lymphoma thymidine kinase (tk(+/-)) gene assay (MOLY), single cell gel electrophoresis (Comet) assay and chromosomal aberration assay in mammalian cells and Ames reverse mutation assay in bacterial system were used as simplified, inexpensive, short-term in vitro screening tests in our laboratory. These compounds are not mutagenic in S. typhimurium TA98 and TA100 strains both in the presence and absence of metabolic activation. Before performing the comet assay, IC20 of Khal was determined the concentration of 25.51 mu g/mL and 21.99 mu g/mL with and without S-9, respectively. In the comet assay, Khal was not induced DNA damage in mouse lymphoma cell line. Also, the mutation frequencies in the Khal-treated cultures were similar to the vehicle controls. It is suggests that Khal is non-mutagenic in MOLY assay. And no clastogenicity was observed in Khal-treated Chinese hamster lung cells. The results of this battery of assays indicate that Khal has no genotoxic potential in bacterial or mammalian cell systems. Therefore, we suggest that Khal, as the optimal candidates with both no genotoxic potential and antibacterial effects must be chosen. English KOREAN SOC TOXICOGENOMICS & TOXICOPROTEOMICS IN-VITRO ANTIMICROBIAL PEPTIDES HALOCYNTHIA-AURANTIUM MUTAGENICITY TEST DNA-DAMAGE CARCINOGENS ASSAY TUNICATE Genotoxicity study on Khal, a halocidin derivative, in bacterial and mammalian cells Article 1 MOLECULAR & CELLULAR TOXICOLOGY, v.2, no.3, pp.151 - 158 MOLECULAR & CELLULAR TOXICOLOGY 2 3 151 158 scie 000243596600001 Biochemistry & Molecular Biology Toxicology Biochemistry & Molecular Biology Toxicology Article IN-VITRO ANTIMICROBIAL PEPTIDES HALOCYNTHIA-AURANTIUM MUTAGENICITY TEST DNA-DAMAGE CARCINOGENS ASSAY TUNICATE Khal Halocynthia aurantium Genotoxicity MOLY comet assay chromosome aberration ames reverse mutation assay antibacterial effects