Cho, Young-Dae Choi, Man Ho 2024-01-21T02:30:56Z 2024-01-21T02:30:56Z 2021-09-01 2006-09-20 0253-2964 https://pubs.kist.re.kr/handle/201004/135114 The following study describes the gas chromatography-mass spectrometry (GC-MS) based screening and confirmation analysis of urinary androgenic steroids. Four commercially available solid-phase extraction (SPE) cartridges, Serdolit PAD-1, Sep-pak C-18, amino-propyl, and Oasis HLB, and three different extractive organic solvents, diethyl ether, methyl tert-butyl ether (MTBE), and n-pentane, were tested for sample preparation. Overall, Oasis HLB combined with MTBE extraction provided the highest recoveries in 39 of 46 total androgenic steroids examined and it showed a good extraction yield (>82.1%) for polar steroids, such as metabolites of fluoxymesterone, oxandrolone, and stanozolol, which gave a poor recovery in both n-pentane (9.2-64.3%) and diethyl ether (22.2-73.6%) extractions. All SPE sorbents tested showed potential, because they were efficient in extraction for most or selective steroids. When applied to positive urine samples based on the results obtained, the present method allowed selective and sensitive analysis for detection of urinary androgenic steroids. The experiments showed that the high-resolution MS method is clearly more efficient than the low-resolution MS technique for the detection of many urinary steroids. However, comprehensive sample clean-up procedures also might be needed especially in confirmation analysis to increase detectability. English KOREAN CHEMICAL SOC SOLID-PHASE EXTRACTION ANABOLIC-STEROIDS IDENTIFICATION DERIVATIZATION METABOLITES ESTROGENS PLASMA Alternative sample preparation techniques in gas chromatographic-mass spectrometric analysis of urinary androgenic steroids Article 1 BULLETIN OF THE KOREAN CHEMICAL SOCIETY, v.27, no.9, pp.1315 - 1322 BULLETIN OF THE KOREAN CHEMICAL SOCIETY 27 9 1315 1322 scie scopus kci ART001023558 000241448300010 2-s2.0-33749016199 Chemistry, Multidisciplinary Chemistry Article SOLID-PHASE EXTRACTION ANABOLIC-STEROIDS IDENTIFICATION DERIVATIZATION METABOLITES ESTROGENS PLASMA solid-phase extraction steroids mass spectrometry doping control