Park, Hee-Deung Wells, George F. Bae, Hyokwan Criddle, Craig S. Francis, Christopher A. 2024-01-21T02:35:47Z 2024-01-21T02:35:47Z 2021-08-31 2006-08 0099-2240 https://pubs.kist.re.kr/handle/201004/135314 We report molecular evidence that ammonia-oxidizing archaea (AOA) occur in activated sludge bioreactors used to remove ammonia from wastewater. Using PCR primers targeting archaeal ammonia monooxygenase subunit A (amoA) genes, we retrieved and compared 75 sequences from five wastewater treatment plants operating with low dissolved oxygen levels and long retention times. All of these sequences showed similarity to sequences previously found in soil and sediments, and they were distributed primarily in four major phylogenetic clusters. One of these clusters contained virtually identical amoA sequences obtained from all five activated sludge samples (from Oregon, Wisconsin, Pennsylvania, and New Jersey) and accounted for 67% of all the sequences, suggesting that this AOA phylotype may be widespread in nitrifying bioreactors. English AMER SOC MICROBIOLOGY OCEAN POPULATIONS PHYLOGENY DIVERSITY NITRITE AMOA Occurrence of ammonia-oxidizing archaea in wastewater treatment plant bioreactors Article 10.1128/AEM.00402-06 1 APPLIED AND ENVIRONMENTAL MICROBIOLOGY, v.72, no.8, pp.5643 - 5647 APPLIED AND ENVIRONMENTAL MICROBIOLOGY 72 8 5643 5647 scie scopus 000239780400065 Biotechnology & Applied Microbiology Microbiology Biotechnology & Applied Microbiology Microbiology Article OCEAN POPULATIONS PHYLOGENY DIVERSITY NITRITE AMOA ammonia Oxidizing archaea amoA gene phylogenetic analysis