Kim, Deok-Ho Kim, Pilnam Song, Inseon Cha, Jae Min Lee, Sang Ho Kim, Byungkyu Suh, Kahp Y. 2024-01-21T03:01:39Z 2024-01-21T03:01:39Z 2021-09-01 2006-06-06 0743-7463 https://pubs.kist.re.kr/handle/201004/135404 We introduce well-defined nanopillar arrays of a poly(ethylene glycol) ( PEG) hydrogel as a cell culture platform to guide a 3D construct of primary rat cardiomyocytes in vitro for potential tissue engineering applications. Ultraviolet (UV)-assisted capillary lithography was used to fabricate highly uniform similar to 150 nm PEG pillars with similar to 400 nm height. It was found that cell adhesion was significantly enhanced on PEG nanopillars (132 +/- 29 cells/mm(2)) compared to that on the bare PEG control (39 +/- 17 cells/mm(2)) (p < 0.05) but substantially reduced compared to that on the glass control (502 +/- 45 cells/mm(2)) (p < 0.01). Furthermore, in colonizing cardiomyocytes, the nanopillars stimulated self-assembled aggregates among the contacting cells with 3D growth, which is a unique feature for nanopatterned PEG hydrogels as a cell culture substrate. The 3D-grown cardiomyocytes retained their conductive and contractile properties, as evidenced by the observation of beating cardiomyocytes with robust action potential generation. English AMER CHEMICAL SOC POLY(ETHYLENE GLYCOL) PROTEIN ADSORPTION CELL ADHESIVE HYDROGELS TOPOGRAPHY MORPHOLOGY SURFACES SCAFFOLD NANOTOPOGRAPHY FABRICATION Guided three-dimensional growth of functional cardiomyocytes on polyethylene glycol nanostructures Article 10.1021/la060283u 1 LANGMUIR, v.22, no.12, pp.5419 - 5426 LANGMUIR 22 12 5419 5426 scie scopus 000237921100036 2-s2.0-33745445953 Chemistry, Multidisciplinary Chemistry, Physical Materials Science, Multidisciplinary Chemistry Materials Science Article POLY(ETHYLENE GLYCOL) PROTEIN ADSORPTION CELL ADHESIVE HYDROGELS TOPOGRAPHY MORPHOLOGY SURFACES SCAFFOLD NANOTOPOGRAPHY FABRICATION