Kim, Hyunki Kang, Hyun Ju You, Kwon Tae Kim, Se Hoon Lee, Kang Young Kim, Tae Il Kim, Chul Song, Si Young Kim, Hye-Jung Lee, Cheolju Kim, Hoguen 2024-01-21T03:03:23Z 2024-01-21T03:03:23Z 2021-09-01 2006-06 1615-9853 https://pubs.kist.re.kr/handle/201004/135476 The purpose of this study was to analyze altered protein expression in cancer tissues and determine its relationship to prognosis in colorectal carcinomas. We performed proteomic expression analysis on 14 colorectal carcinomas and matched nontumorous colonic mucosa by 2-DE and MALDI-TOF-MS. Comparative analysis of the respective spot patterns on 2-DE showed 14 spots that were markedly changed in the colorectal carcinomas. Among them, selenium-binding protein 1 (SELENBP1) was markedly decreased in 12 (85%) carcinomas. The reduced expression of SELENBP1 was further supported by Western blot analysis and immunohistochemistry. Suppression of SELENBP1 was further analyzed in another eight-paired adenomas and carcinomas from the same patients using Western blot analysis and immunohistochemistry, and revealed that one adenoma and seven carcinomas exhibited markedly reduced SELENBP1 expression. Patients with low levels of SELENBP1 expression had significantly lower overall survival rates (72 vs. 85%, p = 0.021) among the 240 stages II and III colorectal carcinomas by using tissue microarray analysis. Our findings indicate that suppression of SELENBP1 is a frequent and late event in colorectal carcinogenesis, and may contribute to the rapid progression of colorectal carcinoma. English WILEY PROTEOMIC ANALYSIS LUNG-CANCER HEPATOCELLULAR-CARCINOMA GEL-ELECTROPHORESIS BREAST-CANCER EXPRESSION IDENTIFICATION PREVENTION SUPPLEMENTATION ADENOCARCINOMA Suppression of human selenium-binding protein 1 is a late event in colorectal carcinogenesis and is associated with poor survival Article 10.1002/pmic.200500629 1 PROTEOMICS, v.6, no.11, pp.3466 - 3476 PROTEOMICS 6 11 3466 3476 scie scopus 000238333400021 2-s2.0-33745076486 Biochemical Research Methods Biochemistry & Molecular Biology Biochemistry & Molecular Biology Article PROTEOMIC ANALYSIS LUNG-CANCER HEPATOCELLULAR-CARCINOMA GEL-ELECTROPHORESIS BREAST-CANCER EXPRESSION IDENTIFICATION PREVENTION SUPPLEMENTATION ADENOCARCINOMA biomarker colorectal carcinoma prognosis selenium-binding protein 1