Babu, CVS Chung, BC Lho, DS Yoo, YS 2024-01-21T03:06:06Z 2024-01-21T03:06:06Z 2021-09-01 2006-04-14 0021-9673 https://pubs.kist.re.kr/handle/201004/135586 Immunoassays are commonly used in bioresearch for the detection and quantification of small proteins and macromolecules in biological fluids and other complex matrices. In this report, a competitive immunoassay using capillary electrophoresis (CE) with laser-induced fluorescence was developed for methionine-enkephalin (ME). The method is based on the competitive reaction between the ME and fluorescein conjugated ME (ME-F) with anti-ME antibody, capillary electrophoresis separation of the ME-antibody bound and free ME-F, followed by the laser-induced fluorescence detection of the fluorescent species. With the optimized separation conditions, it was possible to separate the antibody bound and free fluorescien conjugated ME by a capillary electrophoresis-laser-induced fluorescence (CE-LIF) analysis using an uncoated fused-silica capillaries. The results concluded that the assay specificity, selectivity and accuracy were excellent. (c) 2005 Elsevier B.V. All rights reserved. English ELSEVIER SCIENCE BV TANDEM MASS-SPECTROMETRY ZONE-ELECTROPHORESIS PROTEIN-G CHROMATOGRAPHY NEUROPEPTIDES ANTIBODY ASSAY SEPARATION PITUITARY LIGAND Capillary electrophoretic competitive immunoassay with laser-induced fluorescence detection for methionine-enkephalin Article 10.1016/j.chroma.2005.06.031 1 JOURNAL OF CHROMATOGRAPHY A, v.1111, no.2, pp.133 - 138 JOURNAL OF CHROMATOGRAPHY A 1111 2 133 138 scie scopus 000236656300005 2-s2.0-33645229999 Biochemical Research Methods Chemistry, Analytical Biochemistry & Molecular Biology Chemistry Article; Proceedings Paper TANDEM MASS-SPECTROMETRY ZONE-ELECTROPHORESIS PROTEIN-G CHROMATOGRAPHY NEUROPEPTIDES ANTIBODY ASSAY SEPARATION PITUITARY LIGAND CE-LIF competitive immunoassay methionine-enkephalin