Ahn, KS Noh, EJ Cha, KH Kim, YS Lim, SS Shin, KH Jung, SH 2024-01-21T03:06:10Z 2024-01-21T03:06:10Z 2021-09-01 2006-04-11 0024-3205 https://pubs.kist.re.kr/handle/201004/135589 In the present study, we investigated anti inflammatory effects of six flavonoids isolated from the rhizomes of Belamcanda chinensis (Iridaceae) in RAW 264.7 macrophages. The results indicated that irigenin concentration dependently inhibited lipopolysaccharide (LPS)-induced nitric oxide (NO) and prostaglandin (PG) E-2 production. Furthermore, this compound inhibited the expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX)-2 proteins and mRNAs without an appreciable cytotoxic effect. Treatment of the transfectant RAW 264.7 cells with irigenin reduced the level of nuclear factor-kappa B (NF-kappa B) activity, also effectively lowered NF-kappa B binding measured by electrophoretic mobility shift assay (EMSA), which was associated with decreased p65 protein levels in the nucleus. On the basis of the above data, we suggest that the effect of irigenin in decreasing LPS-induced NO and PGE(2) synthesis is due to diminish the mRNA and protein expression of iNOS and COX-2, respectively, also may be due to under the suppression of NF-kappa B activation. Therefore, irigenin isolated from the rhizomes of Belamcanda chinensis could be offered as a leading compound for antiinflammation. (c) 2005 Elsevier Inc. All rights reserved. English PERGAMON-ELSEVIER SCIENCE LTD NF-KAPPA-B TRANSCRIPTION FACTOR MAMMALIAN-CELLS ISOFLAVONOIDS LIPOPOLYSACCHARIDE EXPRESSION INDUCTION SYNTHASE TISSUES Inhibitory effects of Irigenin from the rhizomes of Belamcanda chinensis on nitric oxide and prostaglandin E-2 production in murine macrophage RAW 264.7 cells Article 10.1016/j.lfs.2005.09.041 1 LIFE SCIENCES, v.78, no.20, pp.2336 - 2342 LIFE SCIENCES 78 20 2336 2342 scie scopus 000236633700007 2-s2.0-33645382382 Medicine, Research & Experimental Pharmacology & Pharmacy Research & Experimental Medicine Pharmacology & Pharmacy Article NF-KAPPA-B TRANSCRIPTION FACTOR MAMMALIAN-CELLS ISOFLAVONOIDS LIPOPOLYSACCHARIDE EXPRESSION INDUCTION SYNTHASE TISSUES Belamcanda chinensis Iridaceae inducible nitric oxide synthase cyclooxygenase-2 nuclear factor-kappa B