Kim, JH Kim, YS Kim, S Park, JH Kim, K Choi, K Chung, H Jeong, SY Park, RW Kim, IS Kwon, IC 2024-01-21T03:32:45Z 2024-01-21T03:32:45Z 2021-09-02 2006-03-10 0168-3659 https://pubs.kist.re.kr/handle/201004/135657 Self-assembled nanoparticles based on hydrophobically modified glycol chitosan (HGC) were prepared as a carrier for paclitaxel. HGC conjugates were prepared by chemically linking 5 beta-cholanic acid to glycol chitosan chains using 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide chemistry. In phosphate-buffered saline (PBS; pH 7.4), the synthesized HGC conjugates formed nano-sized particles with a diameter of 200 nm and exhibited high thermodynamic stability as reflected by their low critical aggregation concentration (0.03 mg/ml). Paclitaxel was efficiently loaded into HGC nanoparticles up to 10 wt.% using a dialysis method. The paclitaxel-loaded HGC (PTX-HGC) nanoparticles were 400 nm in diameter and were stable in PBS for 10 days. These PTX-HGC nanoparticles also showed sustained release of the incorporated of paclitaxel (80% of the loaded dose was released in 8 days at 37 degrees C in PBS). Owing to sustained release, the PTX-HGC nanoparticles were less cytotoxic to B16F10 melanoma cells than free paclitaxel formulated in Cremophor EL. Injection of PTX-HGC nanoparticles into the tail vein of tumor-bearing mice prevented increases in tumor volume for 8 days. Finally, PTX was less toxic to the tumor-bearing mice when formulated in HGC nanoparticles than when formulated with Cremophor EL. (c) 2005 Elsevier B.V. All rights reserved. English ELSEVIER SELF-ASSEMBLED NANOPARTICLES POLYMERIC MICELLES COPOLYMER ADRIAMYCIN AGENT Hydrophobically modified glycol chitosan nanoparticles as carriers for paclitaxel (Reprinted from Journal of Controlled Release, vol 109, pg 1, 2005) Article 10.1016/j.jconrel.2005.12.013 1 JOURNAL OF CONTROLLED RELEASE, v.111, no.1-2, pp.228 - 234 JOURNAL OF CONTROLLED RELEASE 111 1-2 228 234 scie scopus 000236121500026 2-s2.0-33344466821 Chemistry, Multidisciplinary Pharmacology & Pharmacy Chemistry Pharmacology & Pharmacy Reprint SELF-ASSEMBLED NANOPARTICLES POLYMERIC MICELLES COPOLYMER ADRIAMYCIN AGENT hydrophobically modified glycol chitosan paclitaxel nanoparticles in vivo anti-tumor effect