Cha, KH Kim, MD Lee, TH Lim, HK Jung, KH Seo, JH 2024-01-21T03:40:26Z 2024-01-21T03:40:26Z 2021-09-01 2006-02 1017-7825 https://pubs.kist.re.kr/handle/201004/135803 In an attempt to increase production of LK8, an 86-amino-acid kringle fragment of human apolipoprotein(a) with three disulfide linkages, protein disulfide isomerase (PDI) was coexpressed in recombinant Saccharomyces cerevisiae harboring the LK8 gene in the chromosome. Whereas overexpression of the LK8 gene without coexpressing PDI was detrimental to both host cell growth and LK8 production, coexpression of PDI increased the LK8 production level by 2.5-fold in batch cultivation and 5.0-fold in fed-batch cultivation compared with the control strain carrying only the genomic PDI gene. English KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY HUMAN APOLIPOPROTEIN(A) SECRETORY CAPACITY EXPRESSION OVEREXPRESSION GENE ANGIOGENESIS THAUMATIN SELECTION LEVEL Coexpression of protein disulfide isomerase (PDI) enhances production of kringle fragment of human apoliprotein(a) in recombinant Saccharomyces cerevisiae Article 1 JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.16, no.2, pp.308 - 311 JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY 16 2 308 311 scie scopus kci 000235720100022 Biotechnology & Applied Microbiology Microbiology Biotechnology & Applied Microbiology Microbiology Article HUMAN APOLIPOPROTEIN(A) SECRETORY CAPACITY EXPRESSION OVEREXPRESSION GENE ANGIOGENESIS THAUMATIN SELECTION LEVEL apolipoprotein(a) kringle Saccharomyces cerevisidae protein disulfide isomerase