Babu, CVS Lho, DS Yoo, YS 2024-01-21T03:40:40Z 2024-01-21T03:40:40Z 2021-09-02 2006-01-23 0731-7085 https://pubs.kist.re.kr/handle/201004/135807 The developments of bio-analytical methods for analyzing bioactive peptides are of paramount importance. Neuropeptides and their bioactive fragments play a vital role in the regulation of many biological processes and diseases. This paper presents the use of matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) method for monitoring neuropeptides along with their degradation products in plasma samples from cancer patients. The neuropeptides focused in this study were beta-endrophin, substance P, and bradykinin. The method involves the enzyme digestion of the neuroactive peptides followed by MALDI-MS sample preparation and subsequent acquisition of the MS spectral data. The mass spectral profile identifies most of the C-terminal and N-terminal peptides, and the mass accuracy was in the range of -1.68 to 1.46 Da with the mass spectrometer utilised. Analysis of the neuropeptide degradation patterns from the cancer patients were compared with the controls showed similar results. The study reveals that this approach can be used to identify the enzymatic digestion products of protein. (c) 2005 Elsevier B.V. All rights reserved. English ELSEVIER BETA-ENDORPHIN HUMAN-PLASMA DESORPTION IONIZATION CONVERTING-ENZYME SUBSTANCE-P BRADYKININ DEGRADATION CARBOXYPEPTIDASE IDENTIFICATION SEPARATION Monitoring the neuropeptide metabolites by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry Article 10.1016/j.jpba.2005.06.029 1 JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, v.40, no.1, pp.136 - 141 JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 40 1 136 141 scie scopus 000234940300019 2-s2.0-30344442129 Chemistry, Analytical Pharmacology & Pharmacy Chemistry Pharmacology & Pharmacy Article BETA-ENDORPHIN HUMAN-PLASMA DESORPTION IONIZATION CONVERTING-ENZYME SUBSTANCE-P BRADYKININ DEGRADATION CARBOXYPEPTIDASE IDENTIFICATION SEPARATION neuropeptides neuropeptide fragments metabolites MALDI-TOF-MS cancer