Sang, BI Lee, WK Hori, K Unno, H 2024-01-21T03:43:30Z 2024-01-21T03:43:30Z 2021-09-02 2006-01 0959-3993 https://pubs.kist.re.kr/handle/201004/135863 Poly(3-hydroxybutyrate) [P(3HB)] depolymerase was purified from a poly(3-hydroxybutyrate-co-3-hydroxyvalerate) [P(3HB-co-3HV)]-degrading fungus, Paecilomyces lilacinus F4-5 by hydrophobic and ion exchange column chromatography, and showed a molecular mass of 45 kDa. The optimum temperature and pH of the P(3HB) depolymerase were 50 degrees C and 7.0, respectively. The enzyme was stable for at least 30 min at temperatures below 40 degrees C, while the activity abruptly decreased over 55 degrees C. Enzymatic P(3HB-co-3HV) degradation showed a similar degradation pattern to that of film overlaid by fungal hyphae. It reflects that the fungal degradation of P(3HB-co-3HV) in soil is mainly caused by extracellular depolymerases. English SPRINGER IN-SITU BIODEGRADATION Purification and characterization of fungal poly(3-hydroxybutyrate) depolymerase from Paecilomyces lilacinus F4-5 and enzymatic degradation of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) film Article 10.1007/s11274-005-5773-0 1 WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, v.22, no.1, pp.51 - 57 WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY 22 1 51 57 scie scopus 000235486400009 2-s2.0-33344466349 Biotechnology & Applied Microbiology Biotechnology & Applied Microbiology Article IN-SITU BIODEGRADATION biodegradation fungi P(3HB) depolymerase P(3HB-co-3HV)-degrading enzyme