Rhee, KH Lee, KS Priyadarshi, A Kim, EE Hwang, KY 2024-01-21T04:09:00Z 2024-01-21T04:09:00Z 2021-09-02 2005-11 2053-230X https://pubs.kist.re.kr/handle/201004/136031 Fatty acid-CoA racemase plays an important role in the beta-oxidation of branched-chain fatty acids and fatty-acid derivatives as it catalyzes the conversion of several (2R)-branched-chain fatty acid-CoAs to their (2S)-stereoisomers. Fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv has been purified to homogeneity and crystallized by the hanging-drop vapour-diffusion method with polyethylene glycol 4000 as precipitant. The crystals belong to the trigonal space group P3(1) or P3(2), with unit-cell parameters a = b = 109.56, c = 147.97 angstrom. The asymmetric unit contains six monomers, corresponding to a V-M value of 2.15 angstrom (3) Da(-1). A complete native data set has been collected at 2.7 angstrom resolution using a synchrotron-radiation source. English INT UNION CRYSTALLOGRAPHY PEROXISOMAL DISORDERS PHYTANIC ACID ALPHA-OXIDATION DIFFRACTION Crystallization and preliminary X-ray crystallographic studies of fatty acid-CoA racemase from Mycobacterium tuberculosis H37Rv Article 10.1107/S1744309105034330 1 ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, v.61, pp.1017 - 1019 ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS 61 1017 1019 scie scopus 000232890200017 2-s2.0-33744496308 Biochemical Research Methods Biochemistry & Molecular Biology Biophysics Crystallography Biochemistry & Molecular Biology Biophysics Crystallography Article PEROXISOMAL DISORDERS PHYTANIC ACID ALPHA-OXIDATION DIFFRACTION Racemase fatty acid-CoA racemase Mycobacterium tuberculosis α-oxidation β-oxidation