Jung, Hyun Jung Ahn, K.-D. Han, Dong Keun Ahn, D.-J. 2024-01-21T04:13:03Z 2024-01-21T04:13:03Z 2021-09-02 2005-10 1598-5032 https://pubs.kist.re.kr/handle/201004/136108 The interactions between the surface of scaffolds and specific cells play an important role in tissue engineering applications. Some cell adhesive ligand peptides including Arg-Gly-Asp (RGD) have been grafted into polymeric scaffolds to improve specific cell attachment. In order to make cell adhesive scaffolds for tissue regeneration, biodegradable nonporous poly(L-lactic acid) (PLLA) films were prepared by using a solvent casting technique with chloroform. The hydrophobic PLLA films were surface-modified by Argon plasma treatment and in situ direct acrylic acid (AA) grafting to get hydrophilic PLLA-g-PAA. The obtained carboxylic groups of PLLA-g-PAA were coupled with the amine groups of Gly-Arg-Asp-Gly (GRDG, control) and GRGD as a ligand peptide to get PLLA-g-GRDG and PLLA-g-GRGD, respectively. The surface properties of the modified PLLA films were examined by various surface analyses. The surface structures of the PLLA films were confirmed by ATR-FTIR and ESCA, whereas the immobilized amounts of the ligand peptides were 138-145 pmol/cm2. The PLLA surfaces were more hydrophilic after AA and/or RGD grafting but their surface morphologies showed still relatively smoothness. Fibroblast adhesion to the PLLA surfaces was improved in the order of PLLA control < PLLA-g-PAA=PLLA-g-GRDG < PLLA-g-GRGD, indicating that PLLA-g-GRGD has the highest cell adhesive property. English Polymer Society of Korea Surface characteristics and fibroblast adhesion behavior of RGD-immobilized biodegradable PLLA films Article 10.1007/bf03218479 1 Macromolecular Research, v.13, no.5, pp.446 - 452 Macromolecular Research 13 5 446 452 scie scopus kci ART000975575 000233052700012 2-s2.0-27744590187 Polymer Science Polymer Science Article TISSUE PEPTIDES POLYMERS BIOMATERIALS DENSITY STERILIZATION COPOLYMER WELL Direct AA grafting Fibroblast adhesion Plasma treatment PLLA film RGD immobilization Tissue engineering