Kim, Youn-Jung Yun, Hye-Jung Ryu, Jae-Chun 2024-01-21T04:13:32Z 2024-01-21T04:13:32Z 2021-09-03 2005-09-30 1738-642X https://pubs.kist.re.kr/handle/201004/136116 Di (n-ethylhexyl) phthalate (DEHP) is thought to mimic estrogens in their action, and are called endocrine disrupting chemicals. DEHP is used in numerous consumer products, especially those made of flexible polyvinyl chloride and have been reported to be weakly estrogenic. In this study, DEHP were tested for estrogenic properties in vitro models and with microarray analysis. First, the E-screen assay was used to measure the proliferation of DEHP in MCF-7 cells, a human breast cancer cell line. DEHP induced an increase in MCF-7 cell proliferation at concentration of 10(-4) M. Second, we carried out a microarray analysis of MCF-7 cells treated with DEHP using human c-DNA microarray including 401 endocrine system related genes. Of the genes analyzed, 60 genes were identified showing significant changes in gene expression resulting from DEHP. Especially, 4 genes were repressed and 4 genes were induced by DEHP compared to 17 beta-estradiol. Among these genes, trefoil factor 3 (intestinal), breast cancer 1, early onset and CYP1B1 are involved in estrogen metabolism and regulation. Therefore it suggests that these genes may be associated with estrogenic effect of the DEHP on transcriptional level. The rationale is that, as gene expression is a sensitive end-point, alterations of these genes may act as useful biomarkers to define more precisely the nature and level of exposure to kinds of phthalates. English KOREAN SOC TOXICOGENOMICS & TOXICOPROTEOMICS ENVIRONMENTAL-POLLUTANTS PHTHALATE-ESTERS DNA MICROARRAY LINE MCF-7 EXPRESSION DI(2-ETHYLHEXYL)PHTHALATE TOXICITY BREAST ASSAY RECEPTORS Toxicogenomic analysis and identification of estrogen responsive genes of di (n-ethylhexyl) phthalate in MCF-7 cells Article 1 MOLECULAR & CELLULAR TOXICOLOGY, v.1, no.3, pp.149 - 156 MOLECULAR & CELLULAR TOXICOLOGY 1 3 149 156 scie 000243595800002 Biochemistry & Molecular Biology Toxicology Biochemistry & Molecular Biology Toxicology Article ENVIRONMENTAL-POLLUTANTS PHTHALATE-ESTERS DNA MICROARRAY LINE MCF-7 EXPRESSION DI(2-ETHYLHEXYL)PHTHALATE TOXICITY BREAST ASSAY RECEPTORS di(n-ethylhexyl) phthalate (DEHP) gene expression biomarker estrogen responsive gene endocrine disrupting chemicals