Kim, JY Suh, SI In, MK Paeng, KJ Chung, BC 2024-01-21T04:32:21Z 2024-01-21T04:32:21Z 2021-09-03 2005-09 0253-6269 https://pubs.kist.re.kr/handle/201004/136174 An analytical method was developed for evaluating the cannabidiol (CBD), cannabinol (CBN), and Delta(9)-tetrahydrocannabinol (Delta(9)-THC) level in human hair using gas chromatography-mass spectrometry (GC-MS). Hair samples (50 mg) were washed with isopropyl alcohol and cut into small fragments (< 1 mm). After adding a deuterated internal standard, the hair samples were incubated in 1.0 M NaOH for 10 min at 95 degrees C. The analytes from the resulting hydrolyzed samples were extracted using a mixture of n-hexane-ethyl acetate (75:25, v/v). The extracts were then evaporated, derivatized, and injected into the GC-MS. The recovery ranges of CBD, CBN, and Delta(9)-THC at three concentration levels were 37.9-94.5% with good correlation coefficients (r(2) > 0.9989). The intra-day precision and accuracy ranged from -9.4% to 17.7%, and the inter-day precision and accuracy ranged from -15.5% to 14.5%, respectively. The limits of detection (LOD) for CBD, CBN, and Delta(9)-THC were 0.005, 0.002, and 0.006 ng/mg, respectively. The applicability of this method of analyzing the hair samples from cannabis abusers was demonstrated. English PHARMACEUTICAL SOC KOREA SOLID-PHASE MICROEXTRACTION GC/MS/MS SAMPLES DRUGS NCI Simultaneous determination of cannabidiol, cannabinol, and Delta(9)-tetrahydrocannabinol in human hair by gas chromatography-mass spectrometry Article 10.1007/BF02977406 1 ARCHIVES OF PHARMACAL RESEARCH, v.28, no.9, pp.1086 - 1091 ARCHIVES OF PHARMACAL RESEARCH 28 9 1086 1091 scie scopus kci ART001087174 000232210500016 2-s2.0-26444484290 Chemistry, Medicinal Pharmacology & Pharmacy Pharmacology & Pharmacy Article SOLID-PHASE MICROEXTRACTION GC/MS/MS SAMPLES DRUGS NCI GC-MS hair analysis cannabidiol cannabinol tetrahydrocannabinol