Lee, JW Na, DS Chae, SK Kim, C Kang, JY Ju, BK Lee, H Kim, SU Hwang, CN Lee, SH 2024-01-21T04:34:27Z 2024-01-21T04:34:27Z 2021-09-03 2005-08-16 0743-7463 https://pubs.kist.re.kr/handle/201004/136213 The development of proper biomaterials is critical for the success of cell therapy and modern tissue engineering. Here, we extruded the yolk and remaining inner mass from fertilized zebrafish eggs and used the resulting chorions as a biomaterial for the differentiation and attachment of mouse P19 embryonic carcinoma (EC) cells. Cells inserted into the chorion showed the spontaneous formation of embryoid body due to the repulsive cell adhesion of the chorion and differentiated specifically into neural cells and cardiomyocytes. In contrast, dissolved chorion extracellular matrix (ECM) conferred enhanced cell attachment on it, suggesting that a unique property of the zebrafish chorion with nanoporous structure appears to be responsible for the simple and controllable embryoid formation for stem cell differentiation. These results indicate that chorions from fertilized zebrafish eggs may be used as an extracellular matrix alternative and applied for stem cell differentiation to specific cell lineages. English AMER CHEMICAL SOC EMBRYONAL CARCINOMA-CELLS EXTRACELLULAR-MATRIX NEURONAL DIFFERENTIATION CARDIAC TRANSCRIPTION IN-VITRO EXPRESSION PROTEIN ACID BLASTOCYSTS COMPONENTS Using the chorions of fertilized zebrafish eggs as a biomaterial for the attachment and differentiation of mouse stem cells Article 10.1021/la0504255 1 LANGMUIR, v.21, no.17, pp.7615 - 7620 LANGMUIR 21 17 7615 7620 scie scopus 000231389200001 2-s2.0-33748300736 Chemistry, Multidisciplinary Chemistry, Physical Materials Science, Multidisciplinary Chemistry Materials Science Article EMBRYONAL CARCINOMA-CELLS EXTRACELLULAR-MATRIX NEURONAL DIFFERENTIATION CARDIAC TRANSCRIPTION IN-VITRO EXPRESSION PROTEIN ACID BLASTOCYSTS COMPONENTS stem cell chorion zebrafish differentiation