Park, KH Na, K Kim, SW Jung, SY Park, KH Chung, HM 2024-01-21T04:37:31Z 2024-01-21T04:37:31Z 2021-09-03 2005-08 0141-5492 https://pubs.kist.re.kr/handle/201004/136272 Aggregates (spheroids) of specific cells are often regarded as a better form in artificial organs and mammalian cell bioreactors for improved cell-specific functions. Freshly harvested primary rat hepatocytes, cultivated as spheroids and entrapped in an adhesion molecules of Arg-Gly-Asp (RGD)-conjugated extracellular matrix, have been examined for differentiated morphology and enhanced liver-specific functions. A copolymer of RGD conjugated p(NiPAAm-co-PEG) hydrogel was used to entrap hepatocytes in the forms of spheroids. Over 28 days, entrapped the spheroids had a higher viability and produced albumin and urea at constant rates, while there was slight increase in cell numbers and reduction of albumin secretion in single cell culture in the hydrogel. Hepatocytes cultured in this way are a potentially useful three-dimensional cell system for application in a bioartificial liver device and bioreactor. English SPRINGER LONG-TERM CULTURE GLY-ASP RGD BIOARTIFICIAL LIVER HEPATIC-FAILURE RAT HEPATOCYTES ENTRAPMENT Phenotype of hepatocyte spheroids behavior within thermo-sensitive Poly(NiPAAm-co-PEG-g-GRGDS) hydrogel as a cell delivery vehicle Article 10.1007/s10529-005-8453-0 1 BIOTECHNOLOGY LETTERS, v.27, no.15, pp.1081 - 1086 BIOTECHNOLOGY LETTERS 27 15 1081 1086 scie scopus 000231501100005 2-s2.0-24044527166 Biotechnology & Applied Microbiology Biotechnology & Applied Microbiology Article LONG-TERM CULTURE GLY-ASP RGD BIOARTIFICIAL LIVER HEPATIC-FAILURE RAT HEPATOCYTES ENTRAPMENT Arg-Gly-Asp (RGD) artificial liver hepatocytes hydrogel spheroids