이상협 이한웅 최용수 유명진 정경미 홍석원 김영오 2024-01-21T04:39:17Z 2024-01-21T04:39:17Z 2021-09-06 2005-07 2289-0971 https://pubs.kist.re.kr/handle/201004/136301 The isolated microorganisms, Pseudomonas stutzeri, Raoultella planticola (Klebsiella), Serratia fonticola from petroleum contaminated soil were enriched on benzene, toluene, ethylbenzene, o-xylene as carbon and energy sources, respectively. And the degradation characteristics of BTEX was observed in the mixed BTEX substrates. We found that the BTEX in mixed substrates were degraded more than 50% by three isolated microorganisms. Among three isolated microorganisms, the highest degradation rate was observed in Pseudomonas stutzeri, but the degradation rate was different according to microorganisms. In order to increase the degradation efficiency, we applied the co-culture of isolated three microorganisms. The mixture rate of pseudomonas stutzeri : Raoultella planticola (Klebsiella) : Serratia fonticola was follows ; 1:2:1, 1:1:2, and 2:1:1, respectively. In two co-culture of 1:2:1 and 1:1:2, degradation rate was lower than isolated microorganisms. However, degradation rate became higher than isolated microorganisms and the degradation rate of benzene, toluene, and ethylene was more than 95% in co-culture of 2:1:1. The degradation rate increased through the co-culture of isolated microorganisms, however, the growth rate decreased. This was resulted from the substrate competition between microorganisms. The co-culture of microorganisms is a effective method to increase the degradation efficiency of BTEX and the co-culture mixing rate is a important factor for determination of degradation efficiency. 한국물환경학회 BTEX 분해미생물의 순수분리와 혼합 배양에 따른 기질 분해율 및 미생물 성장률 변화에 관한 연구 A Study on Isolation of BTEX Degrading Microorganism and Variation of BTEX Removal Efficiency and Microorganism Growth Rate According to Co-Culture Article 2 한국물환경학회지, v.21, no.4, pp.347 - 352 한국물환경학회지 21 4 347 352 kci ART001132131 Co-culture Mixed substrate benzene toluene ethylbenzene o- m- p-xylene (BTEX) Co-culture mixing rate Co-culture Mixed substrate benzene toluene ethylbenzene o- m- p-xylene (BTEX) Co-culture mixing rate