Byun, S.-S. Lee, J.Y. Ghil, S.H. Lee, S.S. Lee, J.H. Yook, S.H. Han, D.K. Kim, H.H. Lee, E. 2024-01-21T04:41:13Z 2024-01-21T04:41:13Z 2021-09-02 2005-07 0494-4747 https://pubs.kist.re.kr/handle/201004/136338 Purpose: To investigate the feasibility of using a poly(ε-caprolactone) (PCL) sheet seeded with autologous muscle-derived stem cells as a bladder substitute. Materials and methods: Muscle-derived stem cells were isolated from the gastrocnemius muscle of 9 female Sprague-Dawley rats using a preplate technique, and cultured on a 5×5mm PCL sheet. The sheets were implanted into the mesentery of the rats in an autologous manner. Three rats were sacrificed 2, 4 and 8 weeks after implantation, and the morphological changes were assessed by H&E and immunofluorescence staining including DAPI, myosin heavy chain (MHC) and choline acetyl transferase (CAT). Results: All the rats survived for the scheduled time. A mild inflammatory reaction was observed around the PCL sheet in the postoperative 2-week specimen but this receded with time. Muscle cells on the sheet were observed over the experimental period. The 8-week specimen showed a moderate amount of muscle cells on the sheet, and MHC and CAT immunofluorescence staining showed a positive reaction. The muscle layer was not well organized. Angiogenesis was quite noticable between the sheet and the muscle cells on the 8-week specimen. Conclusions: A PCL sheet seeded with autologous muscle-derived stem cells showed skeletal muscle differentiation on the sheets 8 weeks after mesenteric implantation in an autologous manner. This suggests the feasibility of using a PCL sheet seeded with autologous muscle-derived stem cell as a bladder substitute. Korean 4&apos ,6 diamidino 2 phenylindole choline acetyltransferase myosin heavy chain polycaprolactone angiogenesis animal cell animal experiment animal model animal tissue article autologous stem cell transplantation bladder cell culture cell density cell isolation feasibility study female gastrocnemius muscle immunofluorescence inflammation mesentery muscle cell muscle development nonhuman rat skeletal muscle tissue engineering tissue regeneration Preliminary study of tissue engineered bladder regeneration with poly (ε-caprolactone) (PCL) sheet seeded with autologous muscle-derived stem cell Article 1 Korean Journal of Urology, v.46, no.10, pp.1094 - 1097 Korean Journal of Urology 46 10 1094 1097 scopus ART000974447 2-s2.0-27944461104 Article 4&apos ,6 diamidino 2 phenylindole choline acetyltransferase myosin heavy chain polycaprolactone angiogenesis animal cell animal experiment animal model animal tissue article autologous stem cell transplantation bladder cell culture cell density cell isolation feasibility study female gastrocnemius muscle immunofluorescence inflammation mesentery muscle cell muscle development nonhuman rat skeletal muscle tissue engineering tissue regeneration Atrophy Bladder Regeneration Stem cells