Lee, JS Kim, SH Kim, YJ Akaike, T Kim, SC 2024-01-21T04:41:27Z 2024-01-21T04:41:27Z 2021-09-01 2005-07 1525-7797 https://pubs.kist.re.kr/handle/201004/136341 A surface Of poly(L-lactic acid) (PLLA) was modified by coating with poly [N-p-vinylbenzyl-4-O-beta-D-galactopyranosyl-D-glucoamide] (PVLA), which was employed to improve the hepatocyte adhesion owing to its amphiphilic property and the presence of a hepatocyte recognition motif. We characterized the surface properties through water contact angle, electron spectroscopy for chemical analysis (ESCA), and scanning probe microscopy (SPM). The effect of PVLA coating on the efficiency of hepatocyte adhesion was evaluated by protein assay and optical microscopy. The surface morphology was under the influence of the concentration of PVLA coating solution and it played a critical role in hepatocyte adhesion. It was confirmed that galactose moieties in PVLA, which can bind to the asialoglycoprotein receptor (ASGPR) on hepatocytes, have a more dominant effect on hepatocyte adhesion than enhanced hydrophilicity. We suggest that the PVLA-PLLA system will be a useful method to improve hepatocyte cell seeding and adhesion onto scaffold matrices. English AMER CHEMICAL SOC CARRYING POLYSTYRENE POLYMER-SCAFFOLDS CELL SCAFFOLDS CULTURE LIVER BIOMATERIALS FABRICATION Hepatocyte adhesion on a poly[N-p-vinylbenzyl-4-O-beta-D-galactopyranosyl-D-glucoamide]-coated poly(L-lactic acid) surface Article 10.1021/bm049430y 1 BIOMACROMOLECULES, v.6, no.4, pp.1906 - 1911 BIOMACROMOLECULES 6 4 1906 1911 scie scopus 000230498400015 Biochemistry & Molecular Biology Chemistry, Organic Polymer Science Biochemistry & Molecular Biology Chemistry Polymer Science Article CARRYING POLYSTYRENE POLYMER-SCAFFOLDS CELL SCAFFOLDS CULTURE LIVER BIOMATERIALS FABRICATION Hepatocyte Adhesion PLLA Scaffold tissue engineering