Hwang, JI Shin, KJ Oh, YS Choi, JW Lee, ZW Kim, D Ha, KS Shin, HS Ryu, SH Suh, PG 2024-01-21T04:41:46Z 2024-01-21T04:41:46Z 2021-09-03 2005-06-30 1016-8478 https://pubs.kist.re.kr/handle/201004/136347 Phospholipase C-P (PLC-beta) hydrolyses phosphatidylinositol 4,5-bisphosphate and generates inositol 1,4,5-trisphosphate in response to activation of various G protein-coupled receptors (GPCRs). Using glial cells from knock-out mice lacking either PLC-beta 1 [PLC-PI (-/-) or PLC-beta 3 PLC-beta 3 we examined which isotype of PLC-P participated in the cellular signaling events triggered by thrombin. Generation of inositol phosphates (IPs) was enhanced by thrombin in PLC-beta 1 (-/-) cells, but was negligible in PLC-beta 3 (-/-) cells. Expression of PLC-beta 3 in PLC-beta 3 (-/-) cells resulted in an increase in pertussis toxin (PTx)-sensitive H's in response to thrombin as well as to PARI-specific peptide, while expression of PLC-beta 1 in PLC-beta 1 (-/-) cells did not have any effect on IP generation. The thrombin-induced [Ca2+](i) increase was delayed and attenuated in PLC-beta 3 (-/-) cells, but normal in PLC-beta 1 (-/-) cells. Pertussis toxin evoked a delayed [Ca2+](i) increase in PLC-beta 3 (-/-) cells as well as in PLC-PI (-/-) cells. These results suggest that activation of PLC-beta 3 by pertussis toxin-sensitive G proteins is responsible for the transient [Ca2+](i) increase in response to thrombin, whereas the delayed [Ca2+](i) increase may be due to activation of some other PLC, such as PLC-beta 4, acting via PTx-insensitive G proteins. English KOREAN SOC MOLECULAR & CELLULAR BIOLOGY PROTEIN-COUPLED RECEPTORS SMOOTH-MUSCLE-CELLS GTP-BINDING PROTEIN BETA-GAMMA-SUBUNITS SIGNAL-TRANSDUCTION MUSCARINIC RECEPTOR ALPHA-SUBUNIT ACTIVATION FIBROBLASTS DIVERSITY Phospholipase C-beta 3 mediates the thrombin-induced Ca2+ response in glial cells Article 1 MOLECULES AND CELLS, v.19, no.3, pp.375 - 381 MOLECULES AND CELLS 19 3 375 381 scie scopus kci ART001095722 000230342100010 2-s2.0-27244445213 Biochemistry & Molecular Biology Cell Biology Biochemistry & Molecular Biology Cell Biology Article PROTEIN-COUPLED RECEPTORS SMOOTH-MUSCLE-CELLS GTP-BINDING PROTEIN BETA-GAMMA-SUBUNITS SIGNAL-TRANSDUCTION MUSCARINIC RECEPTOR ALPHA-SUBUNIT ACTIVATION FIBROBLASTS DIVERSITY calcium glial cells inositol phosphates GPCR PLC-beta 3